Journal ArticleDOI
Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse.
TLDR
This system provides high-speed, near-simultaneous acquisition of images of multiple fluorescent probes and of a brightfield channel that observed platelet deposition, tissue factor accumulation and fibrin generation after laser-induced endothelial injury in a single developing thrombus.Abstract:
We have used confocal and widefield microscopy to image thrombus formation in real time in the microcirculation of a living mouse. This system provides high-speed, near-simultaneous acquisition of images of multiple fluorescent probes and of a brightfield channel. Vascular injury is induced with a laser focused through the microscope optics. We observed platelet deposition, tissue factor accumulation and fibrin generation after laser-induced endothelial injury in a single developing thrombus. The initiation of blood coagulation in vivo entailed the initial accumulation of tissue factor on the upstream and thrombus–vessel wall interface of the developing thrombus. Subsequently tissue factor was associated with the interior of the thrombus. Tissue factor was biologically active, and was associated with fibrin generation within the thrombus.read more
Citations
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Journal ArticleDOI
Microparticles in Hemostasis and Thrombosis
A. Phillip Owens,Nigel Mackman +1 more
TL;DR: In cancer, PS+, TF+ MPs are derived from tumors and may serve as a useful biomarker to identify patients at risk for venous thrombosis and this review will summarize the current knowledge of the role of procoagulant MPs in hemostasis and thromBosis.
Journal ArticleDOI
Accumulation of Tissue Factor into Developing Thrombi In Vivo Is Dependent upon Microparticle P-Selectin Glycoprotein Ligand 1 and Platelet P-Selectin
Shahrokh Falati,Qingde Liu,Peter L. Gross,Glenn Merrill-Skoloff,Janet Ing-Yuh Chou,Erik Vandendries,Alessandro Celi,Kevin Croce,Barbara C. Furie,Bruce Furie +9 more
TL;DR: It is proposed that PSGL-1 plays a role in blood coagulation in addition to its known role in leukocyte trafficking by examining thrombus formation in the microcirculation of wild-type and genetically altered mice by real-time in vivo microscopy.
Journal ArticleDOI
The growing complexity of platelet aggregation
TL;DR: The identification of shear-dependent mechanisms of platelet aggregation has raised the possibility that vascular-bed-specific inhibitors of platelets aggregation may be developed in the future that are safer and more effective than existing antiplatelet agents.
Journal ArticleDOI
Adhesion Mechanisms in Platelet Function
TL;DR: The results obtained now permit an attempt to integrate all the available information into a picture that highlights the balanced diversity and synergy of distinct platelet adhesive interactions.
Journal ArticleDOI
Arterial thrombosis—insidious, unpredictable and deadly
TL;DR: Major advances in this field require the identification of specific risk factors for arterial thrombosis in affected individuals and a rethink of the 'one size fits all' approach to antithrombotic therapy.
References
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Journal ArticleDOI
Blood-borne tissue factor: Another view of thrombosis
Peter L.A. Giesen,Ursula Rauch,Bernd Bohrmann,Dorothee Kling,Mercè Roqué,John T. Fallon,Juan J. Badimon,Jacques Himber,Markus A. Riederer,Yale Nemerson +9 more
TL;DR: It is suggested that blood-borne TF is inherently thrombogenic and may be involved in thrombus propagation at the site of vascular injury.
Journal ArticleDOI
Specific Synergy of Multiple Substrate–Receptor Interactions in Platelet Thrombus Formation under Flow
TL;DR: Hemodynamic forces and substrate characteristics define the platelet adhesion pathways leading to thrombogenesis.
Journal ArticleDOI
Targeted Gene Disruption Demonstrates That P-Selectin Glycoprotein Ligand 1 (Psgl-1) Is Required for P-Selectin–Mediated but Not E-Selectin–Mediated Neutrophil Rolling and Migration
Jing Yang,Jing Yang,Takako Hirata,Takako Hirata,Kevin Croce,Kevin Croce,Glenn Merrill-Skoloff,Glenn Merrill-Skoloff,Boris Tchernychev,Boris Tchernychev,Eric Williams,Eric Williams,Robert Flaumenhaft,Robert Flaumenhaft,Barbara C. Furie,Barbara C. Furie,Bruce Furie,Bruce Furie +17 more
TL;DR: Kinetics are consistent with a model in which PSGL-1 is the predominant neutrophil P-selectin ligand but is not a required counterreceptor for E- selectin under in vivo physiological conditions, and indicate that PSGL -1 is required for the early inflammatory responses but not for E–selectin–mediated responses.
Journal ArticleDOI
Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade
TL;DR: TF contains the uncommon tryptophan-lysine-serine (WKS) sequence repeated three times, a sequence the authors find in some serine protease-binding proteins and suggest may represent a functional sequence motif.
Journal ArticleDOI
Isolation of cDNA clones coding for human tissue factor: primary structure of the protein and cDNA.
Eleanor K. Spicer,Renata Horton,Laura J. Bloem,Ronald R Bach,Kenneth R. Williams,Arabinda Guha,Jan P. Kraus,Tsung-Chung Lin,Yale Nemerson,William H. Konigsberg +9 more
TL;DR: The amino acid sequence deduced from the nucleotide sequence of the cDNAs indicates that tissue factor is synthesized as a higher molecular weight precursor with a leader sequence of 32 amino acids, while the mature protein is a single polypeptide chain composed of 263 residues.