Recruitment of the Recombinational Repair Machinery to a DNA Double-Strand Break in Yeast.
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TLDR
ChIP time courses from various mutant strains and additional biochemical studies suggest that Rad52p, Rad55p, and Rad54p each help promote the formation and/or stabilization of the Rad51p nucleoprotein filament and all four Rad proteins associate with homologous donor sequences during strand invasion.About:
This article is published in Molecular Cell.The article was published on 2003-07-01 and is currently open access. It has received 211 citations till now. The article focuses on the topics: RAD52 & Strand invasion.read more
Citations
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Journal ArticleDOI
Mechanism of eukaryotic homologous recombination.
TL;DR: HR accessory factors that facilitate other stages of the Rad51- and Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.
Journal ArticleDOI
Molecular Mechanisms of Ultraviolet Radiation-Induced DNA Damage and Repair
TL;DR: This review deals with UV-induced alterations in DNA and its maintenance by various repair mechanisms that are operative in various organisms with the expense of specific gene products.
Journal ArticleDOI
Recombination proteins in yeast.
TL;DR: Recently studies showing defects in homologous recombination and double-strand break repair in several human cancer-prone syndromes have emphasized the importance of this repair pathway in maintaining genome integrity.
Journal ArticleDOI
Mechanism of homologous recombination: mediators and helicases take on regulatory functions
Patrick Sung,Hannah L. Klein +1 more
TL;DR: This work has shown that mutations in the tumour-suppressor protein BRCA2, which has a mediator function in HR, lead to cancer formation and DNA helicases, such as Bloom's syndrome protein, regulate HR at several levels, in attenuating unwanted HR events and in determining the outcome of HR.
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Recruitment of the INO80 Complex by H2A Phosphorylation Links ATP-Dependent Chromatin Remodeling with DNA Double-Strand Break Repair
TL;DR: It is demonstrated that conversion of the DSB into ssDNA is compromised in arp8 and H2A mutants, which are both deficient for INO80 activity at the site of damage.
References
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Journal ArticleDOI
Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
Jürg Bähler,Jian-Qiu Wu,Mark S. Longtine,Nirav Shah,Amos Mckenzie,Alexander B. Steever,Achim Wach,Peter Philippsen,John R. Pringle +8 more
TL;DR: A straightforward PCR‐based approach to the deletion, tagging, and overexpression of genes in their normal chromosomal locations in the fission yeast Schizosaccharomyces pombe, and a series of plasmids containing the kanMX6 module, which allows selection of G418‐resistant cells and thus provides a new heterologous marker for use in S. pom be.
Journal ArticleDOI
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more
TL;DR: This review encompasses different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
Journal ArticleDOI
Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein
TL;DR: The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair and it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-Stranded form, indicating that RAD 51 can catalyze strand exchange.
Journal ArticleDOI
Targeted disruption of the Rad51 gene leads to lethality in embryonic mice.
Teruhisa Tsuzuki,Y Fujii,Kunihiko Sakumi,Yohei Tominaga,Kazuki Nakao,Mutsuo Sekiguchi,Aizo Matsushiro,Yuichi Yoshimura,MoritaT +8 more
TL;DR: The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast RAD51 genes, both of which are involved in homologous recombination and DNA repair, which means that RAD51 protein plays an essential role in the proliferation of cell.
Journal ArticleDOI
Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae.
J K Moore,James E. Haber +1 more
TL;DR: RAD50 (and by extension XRS2 and MRE11) exerts a much more important role in the insertion-producing pathway of NHEJ repair found in S and/or G2 than in the less frequent deletion events that predominate when HO is expressed only in G1.
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Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more