Journal ArticleDOI
Research on pectin depolymerases in the sixties ‐ a literature review
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This review deals mainly with the literature which has appeared since the discovery of pectin lyases in 1960 and it is emphasized that the choice of substrates and reaction conditions, as well as that of methods of analysis, to a large extent influence the values found for a number of enzyme properties.Abstract:
This review deals mainly with the literature which has appeared since the discovery of pectin lyases in 1960. Those papers have been selected which are reports of investigations carried out with purified enzyme preparations and rather well defined substrates. According to their action on high polymer pectic substances, the enzymes are placed in one of six different groups. Mutual relationships of some of the groups are discussed. It is emphasized that the choice of substrates and reaction conditions, as well as that of methods of analysis, to a large extent influence the values found for a number of enzyme properties. Trends for future research are outlined.read more
Citations
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Effect of the plum curculio, Conotrachelus nenuphar (Herbst), on apple and plum fruit abscission /
TL;DR: It is customary to begin photoing at the upper left hand corner of a large sheet and to continue photoing from left to right in equal sections with a small overlap.
Journal ArticleDOI
Partial Purification and Characterization of an Endo-Polygalacturonase from Monilinia fructicola and its Implication in the Brown Rot Disease of Peaches)
Valerie A. Paynter,Joseph J. Jen +1 more
TL;DR: A polygalacturonase from cultural media of Monilinia fructicola was purified 50-fold with 30% yield by ultrafiltration followed by successive chromatography on Sephadex G-75, Ecteola cellulose, and Biogel P-150 columns, indicating the endo nature of this PG.
References
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Journal ArticleDOI
Splitting of pectin chain molecules in neutral solutions
TL;DR: Observations of the rapid decrease in viscosity and increase in reducing end groups are indicative of a splitting of glycosidic bonds within the pectin chain macromolccules, which strongly indicate the formation of an unsaturated compound.
Journal ArticleDOI
Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.
TL;DR: From the cells of the same organism, an intracellular enzyme wag isolated which has properties similar to those of the extra-cellular polygalacturonic acid trans-eliminase was purified from the culture fluid of Erwinia carolovora.
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