Journal ArticleDOI
Retention of aqueous contents during divalent cation-induced fusion of phospholipid-vesicles
TLDR
The results indicate that the initial events in the fusion of LUV are in general, non-leaky, and the diversity of the relative kinetics of fusion and release in different phospholipid vesicle systems under various ionic conditions is emphasized.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1983-10-12. It has received 59 citations till now. The article focuses on the topics: Vesicle & Lipid bilayer fusion.read more
Citations
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Journal ArticleDOI
Fluorescence method for measuring the kinetics of fusion between biological-membranes
TL;DR: By use of this method, the kinetics of fusion between Sendai virus and erythrocyte ghosts and virus-induced fusion of ghosts were readily revealed and were not related to processes other than fusion.
Journal ArticleDOI
H+- and Ca2+-induced fusion and destabilization of liposomes
TL;DR: It is shown that H+ causes lipid mixing in this pH range but not mixing of aqueous contents, which affirms the necessity of using both aqueously space and lipid bilayer assays to comprehend the fusion event between two liposomes.
Journal ArticleDOI
Insertion of poly(ethylene glycol) derivatized phospholipid into pre-formed liposomes results in prolonged in vivo circulation time
Paul S. Uster,Theresa M. Allen,Barbra E. Daniel,Cecilia J. Mendez,Mary S. Newman,Zhu George Z +5 more
TL;DR: Transfer of MPEG1900‐DSPE from micellar phase to pre‐formed liposomes imparts long in vivo circulation half‐life to an otherwise rapidly cleared lipid composition and suggests a simple and economical way to impart site‐specific targeting to a variety of liposome delivery systems.
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Chemistry and biology of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: fluorescent probes of biological and model membranes
TL;DR: Use of NBD-labeled lipids as analogues of natural lipids is critically evaluated and synthetic routes for preparing the lipids, and spectroscopic and ionization properties of these probes are reviewed in this report.
Journal ArticleDOI
Molecular mechanisms of calcium-induced membrane fusion.
TL;DR: A simple molecular model is presented for the mechanism of lipid membrane fusion, consistent with the experimental evidence and incorporating various elements proposed previously.
References
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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Handbook of Physiology.
TL;DR: This is the first volume of the proposed many-sectioned "Handbook" in which the American Physiological Society intends to present comprehensively the entire field of physiology.
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Liposome-Cell Interaction: Transfer and Intracellular Release of a Trapped Fluorescent Marker
TL;DR: When small, unilamellar lipid vesicles containing a high concentration of the fluorescent dye 6-carboxyfluorescein are incubated with either frog retinas or human lymphocytes, fluroescence distributes widely throughout each cell.
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Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals.
TL;DR: The size and shape of the particles as well as the degree of birefringence and the extent to which negative stain penetrates between the lamellae during electron microscopy were characteristic of the various phospholipid species used.
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Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
TL;DR: The name cochleate lipid cylinders is suggested for the spiral structures formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders in phosphatidylserine preparations.