Journal ArticleDOI
Reversal of β-oxidative pathways for the microbial production of chemicals and polymer building blocks.
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TLDR
Recent advancements in β-oxidative strategies are covered and constraints and bottlenecks of this metabolic strategy in comparison to alternative production pathways are discussed.About:
This article is published in Metabolic Engineering.The article was published on 2017-07-01. It has received 44 citations till now. The article focuses on the topics: Metabolic engineering & Fatty acid.read more
Citations
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Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium.
Ingrid Meyer-Cifuentes,Johannes Werner,Nico Jehmlich,Sabine Eva Will,Meina Neumann-Schaal,Başak Öztürk +5 more
TL;DR: The results show that, although there are multiple genes and organisms with the potential to perform each degradation step, only a few are active during biodegradation.
Journal ArticleDOI
Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals.
Qiang Yan,Brian F. Pfleger +1 more
TL;DR: The basic fundamentals of oleochemical synthesis are described and advances since 2013 towards improving performance of heterotrophic microbial cell factories are summarized, showing substantial progress towards producing a subset of these chemicals.
Journal ArticleDOI
Microbial production of short and medium chain esters: Enzymes, pathways, and applications.
Aleksander J. Kruis,Anna C. Bohnenkamp,Constantinos Patinios,Youri M. van Nuland,Mark Levisson,Astrid E. Mars,Corjan van den Berg,Servé W. M. Kengen,Ruud A. Weusthuis +8 more
TL;DR: This review provides a broad overview of natural ester production by microorganisms and focusses on the physical properties of short and medium chain esters, which may simplify downstream processing, while limiting the effects of product toxicity.
Journal ArticleDOI
Key sub-community dynamics of medium-chain carboxylate production
Johannes Lambrecht,Nicolas Cichocki,Florian Schattenberg,Sabine Kleinsteuber,Hauke Harms,Susann Müller,Heike Sträuber +6 more
TL;DR: High-titre medium-chain fatty acid production in a well-established reactor design is possible using complex substrate without the addition of external electron donors, and the pH value influenced the substrate utilisation and product spectrum by shaping the microbial community.
Journal ArticleDOI
Engineered Microorganisms for the Production of Food Additives Approved by the European Union—A Systematic Analysis
TL;DR: This review article is to give a systematic overview on current efforts during construction and application of microbial cell factories for the production of food additives listed in the EU “E numbers” catalog, focused on metabolic engineering strategies of industrially relevant production hosts and discussing current bottlenecks in the underlying metabolic pathways.
References
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Journal ArticleDOI
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
Eric J. Steen,Yisheng Kang,Gregory Bokinsky,Zhihao Hu,Andreas W. Schirmer,Amy McClure,Stephen B. del Cardayre,Jay D. Keasling +7 more
TL;DR: The engineering of Escherichia coli is demonstrated to produce structurally tailored fatty esters (biodiesel), fatty alcohols, and waxes directly from simple sugars, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass.
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Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
TL;DR: The reversal of the β-oxidation cycle was engineered in Escherichia coli and used in combination with endogenous dehydrogenases and thioesterases to synthesize n-alcohols, fatty acids and 3-hydroxy-, 3-keto- and trans-Δ2-carboxylic acids.
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Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli
TL;DR: The genes encoding the four subunits of E. coli ACC were cloned in a single plasmid under the control of a bacteriophage T7 promoter and upon induction of gene expression, the four ACC subunits were overproduced in equimolar amounts.
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Overproduction of free fatty acids in E. coli: implications for biodiesel production.
TL;DR: By introducing four distinct genetic changes into the E. coli genome, this work has engineered an efficient producer of fatty acids and shows a high conversion efficiency of 4.8% of the carbon source into fatty acid products.