Open AccessJournal Article
Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor-growth inhibitor in serum-free medium.
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Normal prokeratinocytes are demonstrated to secrete TGF beta/GI-like molecules into the culture medium and to have specific cell surface receptors for this molecule, and a human squamous cell carcinoma, SCC-25, does not arrest growth when exposed to TGFbeta/GI.Abstract:
Type beta transforming growth factor-growth inhibitor (TGF beta/GI) causes normal human prokeratinocytes to arrest growth predominantly in the G1 phase of the cell cycle within 48 h after log phase cultures are exposed to the factor in serum-free medium. The growth arrest induced by TGF beta/GI is reversible because the cells from treated cultures can be replated into fresh medium and grown into large colonies. Normal prokeratinocytes are demonstrated to secrete TGF beta/GI-like molecules into the culture medium and to have specific cell surface receptors for this molecule. In contrast, a human squamous cell carcinoma, SCC-25, does not arrest growth when exposed to TGF beta/GI. These cells, unlike the normal prokeratinocytes, do not exhibit detectable cell surface receptors for the factor.read more
Citations
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Some recent advances in the chemistry and biology of transforming growth factor-beta.
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Expression cloning of the TGF-β type II receptor, a functional transmembrane serine/threonine kinase
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Transforming Growth Factor-β
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The Transforming Growth Factor-βs
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TL;DR: This chapter summary of current knowledge of the chemistry and complex biology of the growing family of TGF-βs suggests that it has a pivotal control function in many physiological and pathological processes.
References
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Journal ArticleDOI
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Journal Article
Transforming Growth Factor Production by Chemically Transformed Cells
TL;DR: Evidence is presented indicating that the chemically transformed AKR-MCA and C3H/MCA-58 murine cell lines produce "transforming growth factor(s)" capable of inducing a transformed morphology and the ability to grow in soft agar in nontransformed, anchorage-dependent indicator cells.
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