Revisiting TRPC1 and TRPC6 mechanosensitivity
Philip A. Gottlieb,Joost H.A. Folgering,Rosario Maroto,Albert Raso,Thomas G. Wood,Alex Kurosky,Charles L. Bowman,Delphine Bichet,Amanda Patel,Frederick Sachs,Boris Martinac,Owen P. Hamill,Eric Honoré +12 more
TLDR
Although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.Abstract:
This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca2+ permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.read more
Citations
More filters
Journal ArticleDOI
Physiology of cell volume regulation in vertebrates.
TL;DR: Current knowledge regarding the molecular identity of these transport pathways and their regulation by, e.g., membrane deformation, ionic strength, Ca(2+), protein kinases and phosphatases, cytoskeletal elements, GTP binding proteins, lipid mediators, and reactive oxygen species are reviewed.
Journal ArticleDOI
Transient Receptor Potential Channels as Drug Targets: From the Science of Basic Research to the Art of Medicine
Bernd Nilius,Arpad Szallasi +1 more
TL;DR: An overview of the functional properties of mammalian TRP channels is given, their roles in acquired and hereditary diseases are described, and their potential as drug targets for therapeutic intervention is discussed.
Journal ArticleDOI
The Role of Transient Receptor Potential Cation Channels in Ca2+ Signaling
TL;DR: This review focuses on three major tasks of TRP channels: the function of TRp channels as Ca2+ entry channels; the electrogenic actions of TRPs; and (3) TRPs asCa2+ release channels in intracellular organelles.
Journal ArticleDOI
Role of Ion Channels and Transporters in Cell Migration
TL;DR: After presenting general principles by which transport proteins affect cell migration, the role of channels and transporters involved in cell migration is discussed systematically.
Journal ArticleDOI
Renal autoregulation in health and disease.
TL;DR: A combination of vascular and tubular mechanisms, novel to the kidney, provides for high autoregulatory efficiency that maintains RBF and GFR, stabilizes sodium excretion, and buffers transmission of RPP to sensitive glomerular capillaries, thereby protecting against hypertensive barotrauma.
References
More filters
Journal ArticleDOI
Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol.
Thomas Hofmann,Alexander G. Obukhov,Michael Schaefer,Christian Harteneck,Thomas Gudermann,Günter Schultz +5 more
TL;DR: The molecular mechanism of store-depletion-independent activation of a subfamily of mammalian TRPC channels is described and it is found that hTRPC6 is a non-selective cation channel that is activated by diacylglycerol in a membrane-delimited fashion, independently of protein kinases C activated bydiacyl Glycerol.
Journal ArticleDOI
Stretch-activated single ion channel currents in tissue-cultured embryonic chick skeletal muscle.
F. Guharay,Frederick Sachs +1 more
TL;DR: The membrane of tissue‐cultured chick pectoral muscle contains an ionic channel which is activated by membrane stretch, and appears to gather force from a large area of membrane, probably by a cytochalasin‐resistant cytoskeletal network.
Journal ArticleDOI
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
TL;DR: It is demonstrated here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel, and proposed that many TRPC heteromers form diverse receptor-regulated nonselective cation channels in the mammalian brain.
Journal ArticleDOI
TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells
David P. Corey,David P. Corey,Jaime García-Añoveros,Jeffrey R. Holt,Kelvin Y. Kwan,Kelvin Y. Kwan,Shuh Yow Lin,Shuh Yow Lin,Melissa A. Vollrath,Melissa A. Vollrath,Andrea Amalfitano,Eunice L.M. Cheung,Bruce Derfler,Bruce Derfler,Anne Duggan,Gwenaëlle S. G. Géléoc,Paul A. Gray,Matthew P. Hoffman,Heidi L. Rehm,Daniel Tamasauskas,Daniel Tamasauskas,Duan Sun Zhang,Duan Sun Zhang +22 more
TL;DR: Inhibition of TRPA1 protein expression in zebrafish and mouse inner ears inhibits receptor cell function, as assessed with electrical recording and with accumulation of a channel-permeant fluorescent dye.
Journal ArticleDOI
Subunit composition of mammalian transient receptor potential channels in living cells
TL;DR: This work investigates the combinatorial rules of TRPC assembly by pursuing four independent experimental approaches and shows that TRPC2 does not interact with any known TRPC protein andTRPC1 has the ability to form channel complexes together with TRPC4 and TRPC5.