Journal ArticleDOI
RNA fingerprinting and differential display using arbitrarily primed PCR
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TLDR
RNA fingerprinting by arbitrarily primed PCR can be used to detect and clone transcripts that are differentially expressed between cells that have been subject to different environments or developmental programs.About:
This article is published in Trends in Genetics.The article was published on 1995-06-01. It has received 242 citations till now. The article focuses on the topics: Differential display.read more
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DNA fingerprinting in plants: principles, methods, and applications
TL;DR: Amin et al. as discussed by the authors used radioisotopes to measure the diversity of microsatellites in the field of plant DNA collection and preservation of plant Tissue in the Field.
Journal ArticleDOI
cDNA-AFLP Reveals a Striking Overlap in Race-Specific Resistance and Wound Response Gene Expression Profiles
TL;DR: Rapid induction of ACRE genes suggests that they play a pivotal role during plant defense responses, and these genes were also rapidly but transiently induced in leaves in response to other stresses.
Book ChapterDOI
Deletions of the short arm of chromosome 3 in solid tumors and the search for suppressor genes
TL;DR: Though hMLH1 plays an obvious role in the development of specific mismatch repair-deficient cancers, it cannot revert the tumor phenotype and therefore cannot be considered a proper tumor suppressor, and this review discusses the methodological advantages and limitations of the various approaches.
Journal ArticleDOI
Analysis of differential gene expression by display of 3' end restriction fragments of cDNAs
TL;DR: This method produces highly consistent and reproducible patterns, can detect almost all mRNAs in a sample, and can resolve hidden differences such as bands that differ in their sequence but comigrate on a gel, making this a powerful approach to correlate gel patterns with cDNA data bases.
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Unraveling the Secret Lives of Bacteria: Use of In Vivo Expression Technology and Differential Fluorescence Induction Promoter Traps as Tools for Exploring Niche-Specific Gene Expression
TL;DR: By the identification of a large proportion of genes with unknown function, these promoter-trapping studies underscore how little the authors know about the secret lives of bacteria and other microorganisms.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Journal ArticleDOI
Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction
Peng Liang,Arthur B. Pardee +1 more
TL;DR: A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
Journal ArticleDOI
Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes
TL;DR: To assess the distribution and evolutionary conservation of two distinct prokaryotic repetitive elements, consensus oligonucleotides were used in polymerase chain reaction amplification and slot blot hybridization experiments with genomic DNA from diverse eubacterial species.
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Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction
Peng Liang,Arthur B. Pardee +1 more