Rv2686c-Rv2687c-Rv2688c, an ABC Fluoroquinolone Efflux Pump in Mycobacterium tuberculosis
Maria Rosalia Pasca,Paola Guglierame,Fabio Arcesi,Marco Bellinzoni,Edda De Rossi,Giovanna Riccardi +5 more
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TLDR
The Mycobacterium tuberculosis Rv2686c-Rv2687c-rv2688c operon, encoding an ABC transporter, conferred resistance to ciprofloxacin and, to a lesser extent, norfloxACin, moxifloxacIn, and sparfloxAcin to Myc Cobacterium smegmatis.Abstract:
The Mycobacterium tuberculosis Rv2686c-Rv2687c-Rv2688c operon, encoding an ABC transporter, conferred resistance to ciprofloxacin and, to a lesser extent, norfloxacin, moxifloxacin, and sparfloxacin to Mycobacterium smegmatis. The resistance level decreased in the presence of the efflux pump inhibitors reserpine, carbonyl cyanide m-chlorophenylhydrazone, and verapamil. Energy-dependent efflux of ciprofloxacin from M. smegmatis cells containing the Rv2686c-Rv2687c-Rv2688c operon was observed.read more
Citations
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Journal ArticleDOI
Efflux-Mediated Drug Resistance in Bacteria
Xian-Zhi Li,Hiroshi Nikaido +1 more
TL;DR: Fluoroquinolones and β-lactams of the latest generations are likely to select for overproduction mutants of these pumps and make the bacteria resistant in one step to practically all classes of antibacterial agents.
Journal ArticleDOI
Clinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria
TL;DR: This review focuses on chromosomally encoded pumps in bacteria that cause infections in humans, and suggests that resistance nodulation division systems are important in pathogenicity and/or survival in a particular ecological niche.
Journal ArticleDOI
Efflux-mediated antimicrobial resistance
TL;DR: Given the clinical significance of multidrug (and drug-specific) exporters, efflux must be considered in formulating strategies/approaches to treating drug-resistant infections, both in the development of new agents less impacted by efflux and in targeting efflux directly with efflux inhibitors.
Journal ArticleDOI
Synergism between natural products and antibiotics against infectious diseases
TL;DR: The observed synergy and mechanism of action between natural products including flavonoids and essential oils and synthetic drugs in effectively combating bacterial, fungal and mycobacterial infections is described.
Journal ArticleDOI
Efflux-mediated drug resistance in bacteria: an update.
Xian-Zhi Li,Hiroshi Nikaido +1 more
TL;DR: The multifaceted implications of drug efflux transporters warrant novel strategies to combat multidrug resistance in bacteria.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
CmeABC Functions as a Multidrug Efflux System in Campylobacter jejuni
Jun Lin,L.O. Michel,Qijing Zhang +2 more
TL;DR: It is formally established that CmeABC functions as a tripartite multidrug efflux pump that contributes to the intrinsic resistance of C. jejuni to a broad range of structurally unrelated antimicrobial agents.
Journal ArticleDOI
The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis
Martine Braibant,Philippe Gilot +1 more
TL;DR: The inventory and assembly of the typical subunits of the ABC transporters encoded by the complete genome of Mycobacterium tuberculosis found that there is an under-representation of the importers in M. tuberculosis, which may reflect the capacity of this bacterium to synthesize many essential compounds and to grow in the presence of few external nutrients.
Book ChapterDOI
Mechanisms of drug resistance in Mycobacterium tuberculosis
TL;DR: This review presents a meta-analyses of the EMMARM data on antibody titers and its applications in wound care and wound healing and describes the immune responses of mice to EMT.
Journal ArticleDOI
Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis.
Howard Takiff,M Cimino,M C Musso,Torin R. Weisbrod,Rodolfo A. Martinez,M B Delgado,L. Salazar,Barry R. Bloom,William R. Jacobs +8 more
TL;DR: By using the rapid-growing Mycobacterium smegmatis as a model genetic system, a gene was selected that confers low-level FQ resistance when present on a multicopy plasmid, which appears to recognize the hydrophilic FQ, ethidium bromide, acridine, and some quaternary ammonium compounds.
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