Journal ArticleDOI
Salinispora arenicola from temperate marine sediments: new intra-species variations and atypical distribution of secondary metabolic genes
TLDR
Evidence of a much wider geographical distribution and secondary metabolism diversity in this genus than previously projected is provided, and salinosporamide biosynthetic genes, which are highly homologous to those of Bahamas-endemic S. tropica, were detected in several Tosa Bay isolates, making this report the first detection of Salinispora genes in S. arenicola.Abstract:
The obligate marine actinobacterium Salinispora arenicola was successfully cultured from temperate sediments of the Pacific Ocean (Tosa Bay, offshore Kochi Prefecture, Japan) with the highest latitude of 33°N ever reported for this genus. Based on 16S rRNA gene sequence analysis, the Tosa Bay strains are of the same phylotype as the type strain S. arenicola NBRC105043. However, sequence analysis of their 16S-23S rRNA intergenic spacer (ITS) revealed novel sequence variations. In total, five new ITS sequences were discovered and further phylogenetic analyses using gyrase B and rifamycin ketosynthase (KS) domain sequences supported the phylogenetic diversity of the novel Salinispora isolates. Screening of secondary metabolite genes in these strains revealed the presence of KS1 domain sequences previously reported in S. arenicola strains isolated from the Sea of Cortez, the Bahamas and the Red Sea. Moreover, salinosporamide biosynthetic genes, which are highly homologous to those of Bahamas-endemic S. tropica, were detected in several Tosa Bay isolates, making this report the first detection of salinosporamide genes in S. arenicola. The results of this study provide evidence of a much wider geographical distribution and secondary metabolism diversity in this genus than previously projected.read more
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Journal ArticleDOI
The marine actinomycete genus Salinispora: a model organism for secondary metabolite discovery
TL;DR: An overview of the initial discovery of the marine actinomycete genus Salinispora and its development as a model for natural product research illustrates the extraordinary biosynthetic diversity that can emanate from a narrowly defined genus and supports future efforts to explore marine taxa in the search for novel natural products.
Journal ArticleDOI
Drugs and Drug Candidates from Marine Sources: An Assessment of the Current "State of Play"
David J. Newman,Gordon M. Cragg +1 more
TL;DR: It is shown that in a number of disease areas, there are structures originally isolated from marine invertebrates and free-living microbes that have potential, but will need to be "adopted" by pharmaceutical houses in order to maximize their potential.
Journal ArticleDOI
Sioxanthin, a novel glycosylated carotenoid, reveals an unusual subclustered biosynthetic pathway.
TL;DR: Gene sequence homology predicts that the sioxanthin biosynthetic pathway is present in all of the Salinispora as well as other members of the family Micromonosporaceae, and investigations of clustering of carotenoid biosynthesis genes in heterotrophic bacteria show that a non-clustered genome arrangement is more common than previously suggested.
Journal Article
Genome sequencing reveals complex secondary metabolome in the marine actinomycete Salinispora tropica
Daniel W. Udwary,Lisa Zeigler,Ratnakar N. Asolkar,Vasanth R. Singan,Alla Lapidus,William Fenical,Paul R. Jensen,Bradley S. Moore +7 more
TL;DR: This study firmly establishes the genus Salinispora as a rich source of drug-like molecules and importantly reveals the powerful interplay between genomic analysis and traditional natural product isolation studies.
Journal ArticleDOI
Metabolomic Fingerprinting of Salinispora From Atlantic Oceanic Islands.
Anelize Bauermeister,Karen Y. Velasco-Alzate,Tiago Dias,Helena Macedo,Elthon G. Ferreira,Paula C. Jimenez,Paula C. Jimenez,Tito Monteiro da Cruz Lotufo,Norberto Peporine Lopes,Susana P. Gaudêncio,Letícia V. Costa-Lotufo +10 more
TL;DR: This study describes the first occurrence of Salinispora strains in sediments from the Saint Peter and Saint Paul Archipelago in the Atlantic Ocean and investigates the metabolic profiles of these strains by employing mass-spectrometry-based metabolomic approaches, including molecular networking from the Global Natural Products Social Molecular Networking platform.
References
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