Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride.
TLDR
This simple method of specifically introducing a radioactive label into cell surface sialic acids is useful in the study of cell surfaceSialic acid-containing glycoproteins.About:
This article is published in Journal of Biological Chemistry.The article was published on 1977-08-25 and is currently open access. It has received 482 citations till now. The article focuses on the topics: Sialoglycoproteins & Fetuin.read more
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Book ChapterDOI
Chemistry, Metabolism, and Biological Functions of Sialic Acids
TL;DR: This chapter discusses the chemistry, metabolism, and biological functions of sialic acids and the biosynthesis of N-acetylneuraminic acid is briefly reported and more attention is given to the enzyme reactions modifying this compound.
Journal ArticleDOI
Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation
TL;DR: Mild periodate oxidation can prove useful during the early stages of hybridoma screening in order to select for or against anti-carbohydrate antibodies.
Journal ArticleDOI
Fluorescent Analogs of Biomolecular Building Blocks: Design, Properties, and Applications
TL;DR: This article focuses on designing fluorescent probes for the four major families of macromolecular building blocks discussed above, and discusses emissive carbohydrate derivatives, followed byEmissive amino acids, the building blocks of nucleic acids.
Journal ArticleDOI
Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins
Bernd Wollscheid,Damaris Bausch-Fluck,Damaris Bausch-Fluck,Christine Henderson,Robert V. O’Brien,Miriam Bibel,Ralph Schiess,Ruedi Aebersold,Ruedi Aebersold,Julian D. Watts +9 more
TL;DR: A snapshot view of the cell surface N- glycoproteins will enable detection of panels of N-glycoprotein as potential differentiation markers that are currently not accessible by other means.
Journal ArticleDOI
Multifarious roles of sialic acids in immunity
Ajit Varki,Pascal Gagneux +1 more
TL;DR: This overview surveys examples of biological roles of sialic acids in immunity, with emphasis on an evolutionary perspective, given the breadth of the subject.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
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A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.