Journal ArticleDOI
Simple fed-batch technique for high cell density cultivation of Escherichia coli.
TLDR
A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed to maintain carbon-limited growth using a defined medium and cell concentrations of 128 and 148 g per 1 dry cell weight were obtained using glucose or glycerol as carbon source.About:
This article is published in Journal of Biotechnology.The article was published on 1995-02-21. It has received 463 citations till now. The article focuses on the topics: Bioreactor.read more
Citations
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Journal ArticleDOI
Xylose isomerase improves growth and ethanol production rates from biomass sugars for both Saccharomyces pastorianus and Saccharomyces cerevisiae
TL;DR: Examination of the effects of xylose isomerase on cell growth and ethanol production from biomass sugars representative of switch grass and side‐by‐side comparison of S. pastorianus and Saccharomyces cerevisiae demonstrated that the xyloses increased ethanol productivity for both the yeast species by increasing the glucose consumption rate.
Journal ArticleDOI
Effect of high-cell-density fermentation of Candida utilis on kinetic parameters and the shift to respiro-fermentative metabolism
TL;DR: At growth rates above Dr, in a high-cell-density culture, C. utilis acquired a flexible catabolism directed towards alternative fermentation routes, and biomass productivity was higher with molasses than with glucose when the fermenter was operated at high D values.
Patent
Process for large scale production of plasmid dna by e coli fermentation
Michel Chartrain,Laura Kizer Bentley,Barbara Ann Krulewicz,Kristin M. Listner,Wen-Jun Sun,Chanyong Brian Lee +5 more
TL;DR: In this paper, a method for increasing the yield of plasmid DNA production is described, which can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.
Journal ArticleDOI
Production of recombinant ghost bacterial vaccine against streptococcal disease of olive flounder
TL;DR: The results suggest that GBV showed the effectiveness for the protection from the streptococcal infection and had higher potential to induce protective antibodies than FKC vaccine.
Journal ArticleDOI
Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations.
Olga Durany,Philip Bassett,Amanda Weiss,Rocky M. Cranenburgh,Pau Ferrer,Josep López-Santín,Carles de Mas,Julian A. J. Hanak +7 more
TL;DR: The operator-repressor titration system is used to maintain a plasmid during high cell density cultivation and expression of a recombinant protein and the quantity of protein produced was similar to traditional plasmids maintenance systems.
References
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Journal ArticleDOI
Improved oligonudeotide site-directed rautagenesis using M13 vectors
TL;DR: An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides with multiple mismatches to the template, which offers an alternative strategy for construction of multiple mutations over a short region.
Journal ArticleDOI
High cell density cultivation of Escherichia coli at controlled specific growth rate.
D. Riesenberg,V. Schulz,W. A. Knorre,H. D. Pohl,D.J. Korz,Ernst Sanders,A. Ross,W.-D. Deckwer +7 more
TL;DR: The proposed HCDC suppresses generation of inhibitory by-products and the high yield coefficients indicate the economy of the process.
Journal ArticleDOI
Recombinant Protein Expression in High Cell Density Fed-Batch Cultures of Escherichia Coli
L Yee,Harvey W. Blanch +1 more
TL;DR: This review focuses on the use of fed-batch techniques to produce recombinant products in Escherichia coli with high cell concentrations as well as high specific yields of recombinant product.
Journal ArticleDOI
High-cell-density cultivation of Escherichia coli
TL;DR: High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only to allow optimum recombinant protein generation.
Journal ArticleDOI
High density cultivation of biomass in fed-batch system with do-stat
TL;DR: In this article, Escherichia coli and Candida brassicae were cultivated in a fed-batch culture with a DO-stat under the condition that none of the components in the basal medium limited the growth of the microorganism.
Related Papers (5)
Growing E. coli to high cell density--a historical perspective on method development.
Joseph Shiloach,Rephael Fass +1 more