Journal ArticleDOI
Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking
TLDR
A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum.Abstract:
A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum. One analysis run takes 6.5 min including a short organic mobile phase gradient for column regeneration. Concentrations of uric acid, xanthine and hypoxanthine in plasma and serum samples were highly comparable. However, hypoxanthine levels were increased in serum compared to plasma samples due to a prolonged time between serum and blood elements separation. The method was validated for linearity, precision, accuracy, sensitivity and robustness in a similar manner to that for pharmacokinetic data and it is appropriate for physiological and pathophysiological levels of all analytes. The stability of stock standard solutions was verified using spectrophotometric analysis in different conditions. The method is simple and robust with a good precision for the measurement of hypoxanthine, xanthine and uric acid in human plasma and serum.read more
Citations
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Layer-by-layer electrochemical biosensors configuring xanthine oxidase and carbon nanotubes/graphene complexes for hypoxanthine and uric acid in human serum solutions.
TL;DR: Sensing results for the real biological fluidic solutions were finally validated by comparing to those using liquid chromatography-mass spectrometry(LC-MS).
Journal ArticleDOI
Carbon quantum dot coated Fe3O4 hybrid composites for sensitive electrochemical detection of uric acid
Malik Waseem Abbas,Razium Ali Soomro,Razium Ali Soomro,Razium Ali Soomro,Nazar Hussain Kalwar,Mehvish Zahoor,Ahmet Avci,Erol Pehlivan,Keith R Hallam,Magnus Willander +9 more
TL;DR: In this article, the authors explored carbon quantum dots (C-dots) as potential candidates for enhancing the signal sensitivity of an electrochemical sensor devised for biologically important molecule, such as uric acid (UA).
Journal ArticleDOI
Facile fabrication of a non-enzymatic nanocomposite of heteropolyacids and CeO2@Pt alloy nanoparticles doped reduced graphene oxide and its application towards the simultaneous determination of xanthine and uric acid
TL;DR: In this paper, a nonenzymatic nanocomposite of 12-heteropolyacids and CeO2@Pt alloy nanoparticles decorated by reduced graphene oxide (RGO), was successfully developed by a facile and eco-friendly route for simultaneous determination of xanthine (XA) and uric acid (UA).
Journal ArticleDOI
Target-based metabolomics for fast and sensitive quantification of eight small molecules in human urine using HPLC-DAD and chemometrics tools resolving of highly overlapping peaks
TL;DR: The chemometrics multivariate calibration coupled with high performance liquid chromatography-diode array detection analytical strategy was shown as a promising means for fast and sensitive determination of small molecules in human urine and also for fast diagnosis or surveillance in related diseases.
Journal ArticleDOI
A sensitive ratiometric electrochemiluminescence biosensor for hypoxanthine detection by in situ generation and consumption of coreactants
TL;DR: In this article, a ratiometric electrochemiluminescence (ECL) biosensor was constructed for testing hypoxanthine (Hx) using reduced graphene oxide-CdTe quantum dots and luminol.
References
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Journal ArticleDOI
Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis.
TL;DR: It is shown that, at physiological concentrations, urate reduces the oxo-heme oxidant formed by peroxide reaction with hemoglobin, protects erythrocyte ghosts against lipid peroxidation, and protects ERYthrocytes from peroxidative damage leading to lysis.
Journal ArticleDOI
Uric acid: A new look at an old risk marker for cardiovascular disease, metabolic syndrome, and type 2 diabetes mellitus: The urate redox shuttle
Melvin R. Hayden,Suresh C. Tyagi +1 more
TL;DR: Elevations of uric acid > 4 mg/dl should be considered a "red flag" in those patients at risk for cardiovascular disease and should alert the clinician to strive to utilize a global risk reduction program in a team effort to reduce the complications of the atherogenic process resulting in the morbid – mortal outcomes of cardiovascular disease.
Journal ArticleDOI
Renal Transport of Uric Acid: Evolving Concepts and Uncertainties
TL;DR: Understanding the molecular mechanisms of renal urate transport is increasingly important for the practicing clinician as new research unveils their physiologic characteristics, importance in drug action, and genetic association with uric acid levels in human populations.
Journal ArticleDOI
Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography.
TL;DR: A high-performance liquid chromatographic method is described for the separation and quantification of allantoin and oxypurines in plasma and urine samples, indicating a good precision of the method.
Journal ArticleDOI
Interference with clinical laboratory analyses.
M H Kroll,Ronald J. Elin +1 more
TL;DR: An approach to the identification and resolution of an interference problem for the clinical laboratory is proposed and recommendations to manufacturers are made.