Journal ArticleDOI
Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP
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TLDR
A PCR‐RFLP method is described, more efficient, faster and cheaper than those used before, so is recommended for large‐scale processing of field‐collected larval and adult specimens to be identified in malaria vector studies.Abstract:
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.read more
Citations
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Anopheles coluzzii and Anopheles amharicus, new members of the Anopheles gambiae complex.
Maureen Coetzee,Richard H. Hunt,Richard C. Wilkerson,Alessandra della Torre,Mamadou B. Coulibaly,Nora J. Besansky +5 more
TL;DR: Two new species within the Anopheles gambiae complex are here described and named, based on molecular and bionomical evidence.
Journal ArticleDOI
Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection
Anne Boissière,Majoline T. Tchioffo,Dipankar Bachar,Luc Abate,Alexandra Marie,Sandrine E. Nsango,Sandrine E. Nsango,Hamid Reza Shahbazkia,Hamid Reza Shahbazkia,Parfait Awono-Ambene,Elena A. Levashina,Richard Christen,Isabelle Morlais +12 more
TL;DR: Evidence is presented that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections, and the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status.
Journal ArticleDOI
Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms
Federica Santolamazza,Emiliano Mancini,Frédéric Simard,Yumin Qi,Zhijian Tu,Alessandra della Torre +5 more
TL;DR: The approach utilized allowed to develop new easy-to-use co-dominant markers for the analysis of genetic differentiation between M and S-forms and opens new perspectives in the study of the speciation process ongoing within Anopheles gambiae s.
Journal ArticleDOI
Outdoor host seeking behaviour of Anopheles gambiae mosquitoes following initiation of malaria vector control on Bioko Island, Equatorial Guinea.
Michael R. Reddy,Hans J. Overgaard,Simon Abaga,Vamsi P Reddy,Adalgisa Caccone,Anthony E. Kiszewski,Michel A. Slotman +6 more
TL;DR: It is proposed that the long term indoor application of insecticides may have resulted in an adaptive shift toward outdoor host seeking in An.
Journal ArticleDOI
Field-caught permethrin-resistant Anopheles gambiae overexpress CYP6P3, a P450 that metabolises pyrethroids.
Pie Müller,Emma Warr,Bradley J. Stevenson,Patricia Pignatelli,John C. Morgan,Andrew Steven,Alexander E. Yawson,Sara N. Mitchell,Hilary Ranson,Janet Hemingway,Mark J. I. Paine,Martin J. Donnelly +11 more
TL;DR: This is the first study to demonstrate the capacity of a P450 identified in wild A. gambiae to metabolise insecticides, and the findings add to the understanding of the genetic basis of insecticide resistance in wild mosquito populations.
References
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Journal ArticleDOI
Identification of Single Specimens of the Anopheles Gambiae Complex by the Polymerase Chain Reaction
TL;DR: A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of morphologically indistinguishable sibling mosquito species that includes the major vectors of malaria in Africa.
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Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex
TL;DR: Parallel indoor-/outdoor collections of samples from polymorphic populations of arabiensis and gambiae show that adult mosquitoes carrying certain inversion karyotypes do not distribute at random in relation to the human environment, being significantly more frequent in outdoor than in indoor samples, or vice-versa.
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Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex.
TL;DR: Current knowledge on the distribution of the members of the Anopheles gambiae complex is reviewed, especially those belonging to species complexes that contain non-vector species.
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A Polytene Chromosome Analysis of the Anopheles gambiae Species Complex
Mario Coluzzi,Adriana Sabatini,Alessandra della Torre,Maria Angela Di Deco,Vincenzo Petrarca +4 more
TL;DR: In this paper, field-collected specimens of all known taxa in the Anopheles gambiae complex were analyzed on the basis of chromosome inversions with reference to a standard polytene chromosome map.
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Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae
TL;DR: Polytene chromosome studies on the Afrotropical mosquitoes of the Anopheles gambiae complex show that the rearrangements of the banding pattern are all based on paracentric inversions, probably one of the most primitive in the complex.