Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection
Anne Boissière,Majoline T. Tchioffo,Dipankar Bachar,Luc Abate,Alexandra Marie,Sandrine E. Nsango,Sandrine E. Nsango,Hamid Reza Shahbazkia,Hamid Reza Shahbazkia,Parfait Awono-Ambene,Elena A. Levashina,Richard Christen,Isabelle Morlais +12 more
TLDR
Evidence is presented that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections, and the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status.Abstract:
The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the strength of parasite clearance is under genetic control, but little is known about the influence of environmental factors on the transmission success. We present here evidence that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections. A. gambiae mosquitoes collected in natural breeding sites from Cameroon were experimentally challenged with a wild P. falciparum isolate, and their gut bacterial content was submitted for pyrosequencing analysis. The meta-taxogenomic approach revealed a broader richness of the midgut bacterial flora than previously described. Unexpectedly, the majority of bacterial species were found in only a small proportion of mosquitoes, and only 20 genera were shared by 80% of individuals. We show that observed differences in gut bacterial flora of adult mosquitoes is a result of breeding in distinct sites, suggesting that the native aquatic source where larvae were grown determines the composition of the midgut microbiota. Importantly, the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status. This striking relationship highlights the role of natural gut environment in parasite transmission. Deciphering microbe-pathogen interactions offers new perspectives to control disease transmission.read more
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Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Susannah J. Salter,Michael J. Cox,Elena M. Turek,Szymon T. Calus,William O.C.M. Cookson,Miriam F. Moffatt,Paul Turner,Paul Turner,Julian Parkhill,Nicholas J. Loman,Alan W. Walker,Alan W. Walker +11 more
TL;DR: It is demonstrated that contaminating DNA is ubiquitous in commonly used DNA extraction kits and other laboratory reagents, varies greatly in composition between different kits and kit batches, and that this contamination critically impacts results obtained from samples containing a low microbial biomass.
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The gut microbiota of insects - diversity in structure and function.
Philipp Engel,Nancy A. Moran +1 more
TL;DR: Gut bacteria of other insects have also been shown to contribute to nutrition, protection from parasites and pathogens, modulation of immune responses, and communication, and the extent of these roles is still unclear and awaits further studies.
Journal ArticleDOI
Culture of previously uncultured members of the human gut microbiota by culturomics
Jean-Christophe Lagier,Saber Khelaifia,Maryam Tidjani Alou,S. Ndongo,Niokhor Dione,Perrine Hugon,Aurelia Caputo,Frédéric Cadoret,Sory Ibrahima Traore,El hadji Seck,Grégory Dubourg,Guillaume André Durand,Gaël Mourembou,E. Guilhot,Amadou Hamidou Togo,Sara Bellali,Dipankar Bachar,Nadim Cassir,Fadi Bittar,Jeremy Delerce,M. Mailhe,D. Ricaboni,Melhem Bilen,Nicole Prisca Makaya Dangui Nieko,Ndeye Mery Dia Badiane,Camille Valles,Donia Mouelhi,Khoudia Diop,Matthieu Million,Didier Musso,Jônatas Santos Abrahão,Esam I. Azhar,Fehmida Bibi,Muhammad Yasir,Aldiouma Diallo,Cheikh Sokhna,Félix Djossou,Véronique Vitton,Catherine Robert,Jean-Marc Rolain,Bernard La Scola,Pierre-Edouard Fournier,Anthony Levasseur,Didier Raoult +43 more
TL;DR: It is shown that the use of culturomics allows the culture of organisms corresponding to sequences previously not assigned, which doubles the number of species isolated at least once from the human gut.
Journal ArticleDOI
Mosquitoes rely on their gut microbiota for development.
TL;DR: It is suggested that three mosquito species spanning the breadth of the Culicidae depend on their gut microbiome for development, and pyrosequencing of 16S rRNA provides new insights about the acquisition and structure of bacterial communities in mosquitoes.
Journal ArticleDOI
Diversity and function of bacterial microbiota in the mosquito holobiont.
TL;DR: A review of the diversity and functions of mosquito-associated bacteria across multiple variation factors, emphasizing recent findings, is presented.
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