Skin-on-a-chip model simulating inflammation, edema and drug-based treatment
Maierdanjiang Wufuer,Geon Hui Lee,Woojune Hur,Byoungjun Jeon,Byung Jun Kim,Tae Hyun Choi,Sang Hoon Lee +6 more
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TLDR
The development of ‘skin-on-a-chip’ to mimic the structures and functional responses of the human skin and the efficacy of therapeutic drug testing model using this skin chip is evaluated.Abstract:
Recent advances in microfluidic cell cultures enable the construction of in vitro human skin models that can be used for drug toxicity testing, disease study. However, current in vitro skin model have limitations to emulate real human skin due to the simplicity of model. In this paper, we describe the development of 'skin-on-a-chip' to mimic the structures and functional responses of the human skin. The proposed model consists of 3 layers, on which epidermal, dermal and endothelial components originated from human, were cultured. The microfluidic device was designed for co-culture of human skin cells and each layer was separated by using porous membranes to allow interlayer communication. Skin inflammation and edema were induced by applying tumor necrosis factor alpha on dermal layer to demonstrate the functionality of the system. The expression levels of proinflammatory cytokines were analyzed to illustrate the feasibility. In addition, we evaluated the efficacy of therapeutic drug testing model using our skin chip. The function of skin barrier was evaluated by staining tight junctions and measuring a permeability of endothelium. Our results suggest that the skin-on-a-chip model can potentially be used for constructing in vitro skin disease models or for testing the toxicity of cosmetics or drugs.read more
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Organ-on-a-chip devices advance to market
Boyang Zhang,Milica Radisic +1 more
TL;DR: This review traces the history, examines the scientific foundation, envisages the prospect of these renowned organ-on-a-chip technologies, and serves as a guide for new members of this dynamic field to navigate the existing scientific and market space.
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Organ-On-A-Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies.
Samad Ahadian,Robert A. Civitarese,Dawn Bannerman,Mohammad Hossein Mohammadi,Rick Xing Ze Lu,Erika Yan Wang,Locke Davenport-Huyer,Ben Lai,Boyang Zhang,Yimu Zhao,Serena Mandla,Anastasia Korolj,Milica Radisic +12 more
TL;DR: The latest developments in OOC platforms (e.g., liver, skeletal muscle, cardiac, cancer, lung, skin, bone, and brain) are discussed as functional tools in simulating human physiology and metabolism.
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Ping Cui,Sicen Wang +1 more
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Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip
Haitao Liu,Haitao Liu,Yaqing Wang,Yaqing Wang,Kangli Cui,Kangli Cui,Yaqiong Guo,Yaqiong Guo,Xu Zhang,Jianhua Qin +9 more
TL;DR: The remarkable properties of defined hydrogel as proper extracellular matrix that can instruct cellular behaviors are presented and the recent trend where functional hydrogels are integrated into organoids and OOC systems for the construction of 3D tissue models is highlighted.
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Full-thickness human skin-on-chip with enhanced epidermal morphogenesis and barrier function
Gopu Sriram,Gopu Sriram,Massimo Alberti,Yuri Dancik,Bo Wu,Ruige Wu,Zhaoxu Feng,Srinivas Ramasamy,Paul L. Bigliardi,Paul L. Bigliardi,Mei Bigliardi-Qi,Mei Bigliardi-Qi,Zhiping Wang +12 more
TL;DR: It is demonstrated that dynamic perfusion and a fine control of the microenvironment enable improved epidermal morphogenesis and differentiation, and enhanced barrier function, and it is shown that integrated 3D culturing and integrity/permeability testing can be conducted directly on the organ-on-chip device owing to the non-contracting properties of the fibrin-based dermal matrix.
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