Journal ArticleDOI
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes
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TLDR
A factor necessary for the accurate transcription of cloned Xenopus 5S genes in vitro has been isolated from soluble extracts of X. laevis ovaries and the site of action has been investigated using the "footprinting" method of Galas and Schmitz (1978).About:
This article is published in Cell.The article was published on 1980-03-01. It has received 604 citations till now. The article focuses on the topics: General transcription factor & Sp3 transcription factor.read more
Citations
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The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Repetitive zinc‐binding domains in the protein transcription factor IIIA from Xenopus oocytes.
TL;DR: Analysis of the amino acid sequence reveals nine tandem similar units, each consisting of approximately 30 residues and containing two invariant pairs of cysteines and histidines, the most common ligands for zinc in the 7S particle of Xenopus laevis oocytes, which suggests that the protein contains repetitive zinc‐binding domains.
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A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor
TL;DR: One of the complementary DNA clones that was rapidly induced by NGF was found to have a nucleotide sequence that predicts a 54-kilodalton protein with homology to transcriptional regulatory proteins.
Journal ArticleDOI
DNA recognition by Cys2His2 zinc finger proteins.
TL;DR: This review analyzes DNA recognition by Cys2His2 zinc fingers and summarizes progress in generating proteins with novel specificities from fingers selected by phage display.
References
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Journal ArticleDOI
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
David J. Galas,Albert Schmitz +1 more
TL;DR: Estimates indicate that 10-fold sequence-specificity (differential binding constant) could be studied easily using this technique, and the binding of lac repressor to lac operator is visualized by "footprinting" as an example.
Journal ArticleDOI
The use of thin acrylamide gels for DNA sequencing.
Frederick Sanger,Alan Coulson +1 more
TL;DR: Using the plus and minus [l] and the terminator [3] methods, serious variations in the distances between consecutive nucleotide bands in regions of dyad symmetry where base-paired loop structures can form are found, and this can lead to difficulties of interpretation.
Journal ArticleDOI
Selective and accurate initiation of transcription at the ad2 major late promotor in a soluble system dependent on purified rna polymerase ii and dna
TL;DR: Specific transcription initiation at the late promoter is observed with extracts derived from either virus-infected or uninfected KB cells and with class II RNA polymerases isolated from either human calf, murine or amphibian cells.
Journal ArticleDOI
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the region
TL;DR: It is concluded that a control region within the gene directs RNA polymerase III to initiate transcription approximately 50 nucleotides upstream from the 5' border of this region.
Journal ArticleDOI
Separation and characterization of the subunits of ribonucleic acid polymerase.
TL;DR: It is concluded that phosphocellulose enzyme has the structure α2ββ' where the molecular weights of the chains α, β, and β' are 39,000 ± 5%, 155,000 +/- 10%, and 165,000± 10%, respectively.
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A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the region
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
Hugh R.B. Pelham,Donald D. Brown +1 more