Specific peptide-protein interactions in the ribonuclease S' system studied by 13C nuclear magnetic resonance spectroscopy with selectively 13C-enriched peptides.
Reads0
Chats0
About:
This article is published in Journal of Biological Chemistry.The article was published on 1979-05-25 and is currently open access. It has received 22 citations till now. The article focuses on the topics: Nuclear magnetic resonance spectroscopy & Peptide.read more
Citations
More filters
Journal ArticleDOI
Biosynthetic incorporation of 15N and 13C for assignment and interpretation of nuclear magnetic resonance spectra of proteins
TL;DR: The use of isotopic substitution is a time-honoured method for simplifying the nuclear magnetic resonance spectra of biological macromolecules and a number of important conclusions concerning the pKa values of specific residues, the affinity of the protein for various ligands, or dynamic properties of theprotein can be deduced.
Journal ArticleDOI
Ribonuclease S‐peptide as a carrier in fusion proteins
Jin-Soo Kim,Ronald T. Raines +1 more
TL;DR: A sensitive method was developed to detect the fusion protein after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis by its ribonuclease activity following activation with S‐protein.
Book ChapterDOI
12 Pancreatic Ribonuclease
Peter Blackburn,Stanford Moore +1 more
TL;DR: The usefulness of the cytoplasmic inhibitor of RNase to protect RNA in the course of the synthesis of complementary DNA by reverse transcriptase is discussed.
Journal ArticleDOI
Semisynthetic peptides and proteins
TL;DR: Sequence modification through semisynthesis thus provides a flexible route to explore the code of rules whereby primary structure directs higher order properties of folded conformation and biological function of large peptides and proteins.
Journal ArticleDOI
Anion binding and pH-dependent electrostatic effects in ribonuclease
TL;DR: The solvent-accessibility-modified, Tanford-Kirkwood, discrete charge model for electrostatic effects is applied to both ribonuclease A and ribonuklease S, and the predicted response of the active-site histidine pK1/2 values to anion binding are shown to agree with experimental determinations.
References
More filters
Journal ArticleDOI
Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides
Journal ArticleDOI
The Preparation of Subtilisin-modified Ribonuclease and the Separation of the Peptide and Protein Components
TL;DR: Neither S-peptide nor S-protein alone shows appreciable ribonuclease activity, but when these components are mixed in equimolar proportions, almost the full enzymic activity is recovered.
Journal ArticleDOI
The Preparation of Merrifield‐Resins Through Total Esterification With Cesium Salts
TL;DR: The reaction of chloromethylated polystyrene-co-1%-divinylbenzene resin with the cesium salts of N-protected amino acids proceeds fast and without side reactions to give Nprotected amino acyl resin esters free of quaternary ammonium sites or reactive chloride as discussed by the authors.
Journal ArticleDOI
Theory of Nuclear Overhauser Enhancement and 13C–1H Dipolar Relaxation in Proton‐Decoupled Carbon‐13 NMR Spectra of Macromolecules
TL;DR: In this paper, the nuclear Overhauser enhancement (NOE), the spinlattice relaxation time (T1), and the spin spin relaxation time of a 13C nucleus relaxing by a dipolar interaction with one proton under conditions of complete proton decoupling, and without assuming that the extreme narrowing limit applies.
Journal ArticleDOI
Spectrophotometric assay of bovine pancreatic ribonuclease by the use of cytidine 2′:3′-phosphate
TL;DR: The method described here depends on the differences in absorption spectra of cytidine 2':3'-phosphate and cytidine 3'-ph phosphate and is thus similar to that of Richards (1955); the conditions have been worked out to take account of the optical factors.