Journal ArticleDOI
Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.
K. Weber,Th. Bibring,Mary Osborn +2 more
TLDR
Cytoplasmic microtubules, vinblastine-induced paracrystals and the full spectrum of mitotic figures can be visualized by immunofluorescence and show that the tubulin structure has been highly conserved during evolution.About:
This article is published in Experimental Cell Research.The article was published on 1975-10-01. It has received 201 citations till now. The article focuses on the topics: Microtubule & Tubulin.read more
Citations
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Journal ArticleDOI
Taxol stabilizes microtubules in mouse fibroblast cells.
TL;DR: Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.
Journal ArticleDOI
A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.
TL;DR: The results suggest an attachment of polyribosomes to the cytoskeleton via mRNA, which is similar to that in intact cells.
Journal ArticleDOI
Antibody to prekeratin: Decoration of tonofilament-like arrays in various cells of epithelial character
TL;DR: It is concluded that several epithelial cells which are capable of continuous division in culture continuously produce large, balanced amounts of prekeratin-like material which is assembled in tonofilament-like structures.
Book ChapterDOI
Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.
Mary Osborn,Klaus Weber +1 more
TL;DR: This chapter describes the study of the intracellular location of proteins by immunocytochemical procedures, and the methods given are generally applicable for tubulin containing structures and antibodies to tubulin.
Journal ArticleDOI
The detergent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles
Mary Osborn,Klaus Weber +1 more
TL;DR: At least in well spread cells containing a strongly developed system of microfilamentous bundles, the detergent-resistant cytoskeleton includes the nucleus, large amounts of the 58 000 molecular weight protein and the microfilmous bundles.
References
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Journal ArticleDOI
The ultrastructure of a mammalian cell during the mitotic cycle
TL;DR: The precise selection of cells during the various stages of anaphase has made it possible to follow chronologically the morphological features of the initiation of nuclear membrane reformation.
Journal ArticleDOI
Microtubular crystals in mammalian cells
TL;DR: Periwinkle alkaloids in very low concentrations cause an intracytoplasmic sequestration of microtubule protein in the form of symmetrical, microtubular bodies; neither colchicine nor puromycin prevents the formation of these bodies; the latter compound retards crystal growth.
Journal ArticleDOI
Calf and pea histone IV. 3. Complete amino acid sequence of pea seedling histone IV; comparison with the homologous calf thymus histone.
TL;DR: The complete amino acid sequence of pea seedling histone IV has been determined with the tryptic peptides from the maleylated protein and compared to the sequence of calf thymus hist one IV, and similarities and differences are discussed in the context of possible structure-function relationships of histoneIV.
Journal ArticleDOI
Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells
TL;DR: High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibodies show a periodic fluorescence.
Journal ArticleDOI
The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells.
TL;DR: Electron microscopy reveals that living mouse 3T3 cells display a complex array of fibrous structures which are visible with phase contrast, Nomarski and polarized light optics and consist of submembranous bundles of microfilaments located primarily on the attached side of the cells.
Related Papers (5)
Cytoplasmic microtubules in normal and transformed cells in culture: analysis by tubulin antibody immunofluorescence.
Actin antibody: The specific visualization of actin filaments in non-muscle cells
Elias Lazarides,Klaus Weber +1 more