Journal ArticleDOI
Strain-specific detection of introduced Beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers.
Reads0
Chats0
TLDR
Strain-specific molecular markers based on polymerase chain reaction amplification of sequence-characterized amplified regions (SCAR) in combination with dilution plating on semi-selective medium to detect and estimate density of propagules of a commercial strain of B. bassiana (strain GHA) in field samples are developed.About:
This article is published in Journal of Invertebrate Pathology.The article was published on 2003-02-01. It has received 90 citations till now. The article focuses on the topics: RAPD & Bassiana.read more
Citations
More filters
Journal ArticleDOI
Insect pathogens as biological control agents: Back to the future.
Lawrence A. Lacey,David Grzywacz,David I. Shapiro-Ilan,Roger Frutos,M. Brownbridge,Mark S. Goettel +5 more
TL;DR: Current information on development, use and future directions of insect-specific viruses, bacteria, fungi and nematodes as components of integrated pest management strategies for control of arthropod pests of crops, forests, urban habitats, and insects of medical and veterinary importance is presented.
Book ChapterDOI
Laboratory techniques used for entomopathogenic fungi: Hypocreales
TL;DR: This chapter provides an overview of both classical and molecular techniques used to study entomopathogenic hypocrealean fungi and provides information in the form of generalizations as well as via specific examples.
Journal ArticleDOI
Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats.
TL;DR: Using molecular phylogenetic and genotypic information to infer species diversity, reproductive potential and genetic structure of Beauveria occurring within a single arable field and bordering hedgerow in Denmark, it is shown that all five B. bassiana s.s. phylogenetic species possess bipolar outcrossing mating systems.
Journal ArticleDOI
Genetic diversity and population structure among strains of the entomopathogenic fungus, Beauveria bassiana, as revealed by inter-simple sequence repeats (ISSR).
TL;DR: It is suggested that ISSR markers can be used as robust molecular markers for the population genetics, epidemiological and ecological studies of entomopathogenic fungi.
Journal ArticleDOI
Long-term field persistence of Beauveria brongniartii strains applied as biocontrol agents against European cockchafer larvae in Switzerland
TL;DR: Demonstration of long-term persistence of the applied B. brongniartii strains in the fields supported the success of this approach for the biological control of M. melolontha.
References
More filters
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Inhibition and facilitation of nucleic acid amplification.
TL;DR: This review discusses the findings of many studies related to clinical, food, and environmental microbiology, including approaches that have been used to overcome inhibition and facilitate amplification for detection and typing and describes inhibitors and methods that can overcome the attenuation of amplification.
Journal ArticleDOI
Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce
Ilan Paran,Richard W Michelmore +1 more
TL;DR: Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce, providing information on the molecular basis of RAPD markers.
Book
Manual of techniques in insect pathology
TL;DR: Initial Handling and Diagnosis of Diseased Insects and Complementary Techniques--Preparation of Entomopathogens and Diseased Specimens for more detailed study.
Journal ArticleDOI
Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil
Cheryl R. Kuske,Kaysie L. Banton,Dante L. Adorada,Peter C. Stark,Karen K. Hill,Paul J. Jackson +5 more
TL;DR: A small-scale procedure to rapidly extract and purify DNA from soils was developed for in-the-field use and found that combining a hot-detergent treatment with bead mill homogenization gave the highest DNA yields from all three microbial cell types and provided DNA from the broadest range of microbial groups in a natural soil community.