Showing papers in "Journal of Invertebrate Pathology in 2003"

TL;DR: High-fidelity PCR of 16S rRNA sequences was used to identify bacteria associated with worker adults of the honeybee subspecies Apis mellifera capensis and Apis Mellifera scutellata, finding sequences related to Lactobacillus and Bifidobacterium which previously had been identified from other honeybees, as well as sequencesrelated to Bartonella, Gluconacetobacter, Simonsiella/Neisseria, and Serrat

TL;DR: The recency of OTC resistance in P. larvae across this broad survey area underscores the need to manage foulbrood infections carefully and to monitor populations for resistance.

TL;DR: With strong CI and limited effect of fecundity, an introduction of Wolbachia with an initial frequency of 0.4 could result in a 60-80% reduction of transmitting mosquitoes.

TL;DR: Some strains with moderate to high biopesticide activity against Spodoptera frugiperda and Premnotrypes vorax insects were identified, this being important to explore future microbial strategies for the control of these crop pests in the region.

TL;DR: Two greenhouse experiments were conducted to compare the efficacy of entomopathogenic nematodes applied in aqueous suspensions with application in infected cadavers, with superior efficacy observed in the cadaver application.

TL;DR: The thick coating of long-chain wax esters produced by whitefly nymphs affect spore germination of fungal pathogens, but whether they play a significant role in defense against disease is not clear.

TL;DR: Bacillus thuringiensis isolates from different ecological regions and sources of China were analyzed to study the distribution and diversity of cry genes and to detect the presence of novel cry genes.

TL;DR: The results suggest that thermoregulation helped keep fungal growth in check, apparently through the maintenance of hemocyte population levels and the direct inhibition of blastospore propagation by elevated temperatures.

TL;DR: There were significant differences in conidial size, viability, spore production, speed of germination, relative hyphal growth, and temperature sensitivity of Beauveria bassiana and M. anisopliae isolates, which are highly pathogenic to Lygus lineolaris.

TL;DR: Strain-specific molecular markers based on polymerase chain reaction amplification of sequence-characterized amplified regions (SCAR) in combination with dilution plating on semi-selective medium to detect and estimate density of propagules of a commercial strain of B. bassiana (strain GHA) in field samples are developed.

TL;DR: Analysis of ColorBtrus occlusion bodies confirmed that they include both Bt toxin and GFP, yet still incorporate virions, and demonstrated that its speed of action and pathogenicity are strikingly enhanced compared to wild-type virus.

TL;DR: The main response observed was a change in hemocyte type distribution between uninfected and infected oysters and greater tissue-infiltrating hemocytes with increased infections.

TL;DR: The number of N. bombi spores per bee was highly variable among bumble bees within colonies, and accurate estimates could only be obtained by sampling a large proportion of bees in each colony, so whole bee and frass sampling is useful for determining if N.Bombi is present or absent, but not for obtaining accurate estimates of the intensity of N bombi infections.

TL;DR: The infectivity of 4 isolates of Metarhizium anisopliae to puparia of Ceratitis capitata treated as late third-instar larvae in unsterilized soil was investigated in the laboratory under controlled temperature and moisture, indicating the potential of using isolate ICIPE 20 for soil inoculation against pupariating third- instar larva of fruit flies, thus providing a novel alternative to chemical soil application.

TL;DR: Comparative phylogenetic analysis using rDNA shows Nosema grylli to be closely related to another orthopteran microsporidian, Nosema locustae, and to Nosema whitei from the confused flour beetle, Tribolium confusum, which is a sister taxon to these three Nosema species.

TL;DR: The exotic multicolored Asian lady beetle, Harmonia axyridis, became established and recently spread across much of North America and southern Canada and it is hypothesized that low susceptibility of H. axyridi to B. bassiana (found to infect O. v-nigrum) may provide an intraguild advantage to H. Axyridis.

TL;DR: By 10 days after the beginning of the experiments, IJ survival declined to 93, 43, and 28% of levels on day 1 for H. bacteriophora, S. riobrave, and S. carpocapsae, respectively, and for the three treatments, infection rate over time was described by a negative exponential function.

TL;DR: Fungal characteristics other than virulence should be considered for the seasonal colonization approach to termite microbial control.

TL;DR: Microsporidian isolates from five lepidopteran pests were compared by spore morphology, infectivity to S. litura, Western-blot banding patterns, the sequences of small subunit rRNA gene (SSUrRNA sequence), and random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR).

TL;DR: Reduction in food consumption by C. partellus larvae infected with B. bassiana and M. anisopliae may offset the slow speed of kill of the fungi and cause little mortality and mycoses than larvae provided with maize leaves.

TL;DR: The effects of abiotic and biotic soil factors on occurrence of the entomopathogenic fungus Beauveria brongniartii after application at different times of the year were examined in Switzerland, indicating that biotic factors have a greater influence than soil texture.

TL;DR: Experimental infection by bath challenge demonstrated that V. lentus was able to reproduce the skin lesions, colonize the internal organs and induce mortality in healthy octopuses.

TL;DR: The Wolbachia density was higher in the Yokohama strain than the Tsuchiura strain in agreement with the CI levels expressed, but this relationship did not hold in the comparison between the naturally infected and transinfected strains that carried phylogenetically distant WolbachIA.

TL;DR: Spiroplasma kunkelii distribution and infection mechanisms in the intestines and Malpighian tubules of Dalbulus maidis were investigated by transmission electron microscopy and this is the first time that fimbriae and pili have been observed in a mollicutes.

TL;DR: Cloned PCR products amplified from the gut contents of certain mushroom-feeding and wood-ingesting beetles in four families were sequenced and compared with culture results, and phylogenetic analysis of additional clone sequences discovered from passalid beetles showed similarity to members of the Parabasalia, protists known from other wood-editing insects, termites, and wood roaches.

TL;DR: The effects of exposure methods, conidial concentrations, and temperature on mortality, mycosis and sporulation in second instar Chilo partellus cadavers infected by Beauveria bassiana was investigated in laboratory studies.

TL;DR: Starvation of second instar Colorado potato beetle larvae for 24h immediately after treatment with Beauveria bassiana conidia increased susceptibility to the pathogen and subsequent sporulation of cadavers but decreased time to larval death.

TL;DR: The midgut proteases of the Bacillus thuringiensis resistant and susceptible populations of the diamondback moth, Plutella xylostella L. were characterized by using protease specific substrates and inhibitors to characterize protoxins of Cry1Aa and Cry1Ab.

TL;DR: Results from this study suggest that DHCs can be used to assess and characterize inflammation in H. americanus and possibly other crustaceans.