Structural investigations on the 2-keto-3-deoxyoctonate region of lipopolysaccharides.
TLDR
Results were obtained which indicate that the bridge between the polysaccharide portion of lipopolysaccharides and lipid A is formed by a branched KDO trisaccharide whose KDO side chain is randomly substituted by phosphorylethanolamine according to the formula.Abstract:
Mild acid hydrolysis of the glycolipid derived from a Salmonella Rd2 mutant led to the liberation of three major split products which, after separation by electrophoresis, were identified as free 2-keto-3-deoxyoctonate (KDO), KDO-7-phosphorylethanolamine, and l-glycero-α-d-mannoheptosyl-1,5-KDO. By the use of various methods of KDO determinations, and by periodate oxidation performed on the glycolipid, results were obtained which indicate that the bridge between the polysaccharide portion of lipopolysaccharides and lipid A is formed by a branched KDO trisaccharide whose KDO side chain is randomly substituted by phosphorylethanolamine according to the formula —Hep-1,5-KDO-KDO-lipid A | KDO-(phosphorylethanolamine) Analogous results were obtained with P-lipopolysaccharides of other Salmonella mutants.read more
Citations
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Bacterial lipopolysaccharides and innate immunity
TL;DR: The elucidation of structure-activity correlations in LPS and lipid A has not only contributed to a molecular understanding of both immunostimulatory and toxic septic processes, but has also re-animated the development of new pharmacological and immunostIMulatory strategies for the prevention and therapy of infectious and malignant diseases.
Book ChapterDOI
Lipopolysaccharides of Gram-Negative Bacteria
Otto Lüderitz,Marina A. Freudenberg,Chris Galanos,Volker Lehmann,Ernst Th. Rietschel,Derek H. Shaw +5 more
TL;DR: The studies indicate that at least some lipid A activities are not restricted to one specific structure, but are rather connected with general physicochemical properties.
Journal ArticleDOI
Lipopolysaccharide Heterogeneity in Salmonella typhimurium Analyzed by Sodium Dodecyl Sulfate/Polyacrylamide Gel Electrophoresis
E. Tapio Palva,P. Helena Mäkelä +1 more
TL;DR: Electrophoretic analysis of isolated cell envelopes in the presence of sodium dodecyl sulfate revealed at least 40 regularly spaced bands, which indicated major heterogeneity due to differences in chain lengths and a second heterogeneity indicated by the double character of each band.
Journal ArticleDOI
Protein Composition of the Outer Membrane of Salmonella typhimurium: Effect of Lipopolysaccharide Mutations
TL;DR: The protein composition of the outer membrane of Salmonella typhimurium has been analyzed by electrophoresis on slabs of sodium dodecyl sulfate-acrylamide gel and multiple major protein components of theouter membrane were identified.
Book ChapterDOI
The Chemistry and Biological Significance of 3-Deoxy-d-manno-2-Octulosonic Acid (KDO)*
TL;DR: It is found that when oligosaccharides containing reducing-terminal KDO residues are subjected to borohydride reduction prior to Smith degradation, the epimeric pair of oligosACcharides is obtained, containing 3-deoxy- L - ribo - and - arabino -hexonic acid at the end that had formerly been reducing.
References
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A new method for the extraction of R lipopolysaccharides.
TL;DR: A method is described which is specific for the extraction of lipopolysaccharides from R form bacteria, which consists of aqueous phenol, chloroform and petroleum ether.
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Über die Extraktion von Bakterien mit Phenol/Wasser
TL;DR: In this paper, two einfache Verfahren zur extraktion von Bakterien with Phenol/Wasser angegeben are presented. Butt et al.
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