Journal ArticleDOI
Structure and stability of mRNA synthesized by vaccinia virus-encoded bacteriophage T7 RNA polymerase in mammalian cells. Importance of the 5' untranslated leader.
Thomas R. Fuerst,Bernard Moss +1 more
Reads0
Chats0
TLDR
In this paper, the structure and stability of RNA synthesized by bacteriophage T7 RNA polymerase in mammalian cells was analyzed and it was shown that the 5′-terminal double-stranded stem is required to stabilize the nascent RNA against degradation.About:
This article is published in Journal of Molecular Biology.The article was published on 1989-03-20. It has received 142 citations till now. The article focuses on the topics: RNA-dependent RNA polymerase & T7 RNA polymerase.read more
Citations
More filters
Journal ArticleDOI
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
TL;DR: In higher eukaryotes, translation is modulated at the level of initiation by five aspects of mRNA structure: (i) the m7G cap; (ii) the primary sequence or context surrounding the AUG codon; (iii) the position of the Aug codon, i.e. whether or not it s “first”; (iv) secondary structure both upstream and downstream from the AUU; and (v) leader length.
Journal ArticleDOI
Mutations in human dynamin block an intermediate stage in coated vesicle formation
Alexander M. van der Bliek,Alexander M. van der Bliek,Thomas E. Redelmeier,Hanna Damke,Ellen J. Tisdale,Elliot M. Meyerowitz,Sandra L. Schmid +6 more
TL;DR: Results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytotic via clathrin-coated pits.
Journal ArticleDOI
Product review. New mammalian expression vectors.
TL;DR: The RNA polymerases from T7 and related bacteriophages, in conjunction with elements of DNA and RNA viruses, can be used in novel ways for expression of genes in mammalian cells.
Journal ArticleDOI
Vaccinia virus: a tool for research and vaccine development
TL;DR: Rec recombinant vaccinia virus is no longer needed for smallpox immunization, but now serves as a useful vector for expressing genes within the cytoplasm of eukaryotic cells.
Journal ArticleDOI
Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells.
Orna Elroy-Stein,Bernard Moss +1 more
TL;DR: A mouse cell line that constitutively synthesizes the bacteriophage T7 RNA polymerase was constructed and the system provided a unique opportunity to study structural elements of mRNA that affect stability and translation.
References
More filters
Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
Journal ArticleDOI
Vectors for selective expression of cloned DNAs by T7 RNA polymerase
TL;DR: Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribing by T7 RNA polymerases, in vitro or in E. coli cells.
Journal ArticleDOI
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
TL;DR: The complete nucleotide sequence of bacteriophage T7 DNA, 39,936 base-pairs, has been determined and the amino acid sequences and compositions predicted for all of the T7 proteins (except the proteins produced by frameshifting) are given.
Journal ArticleDOI
Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.
Bruce A. White,F C Bancroft +1 more
TL;DR: This technique is quite simple, requires very small amounts of cells or tissue, and permits the simultaneous analysis of multiple samples, which should be quite useful for studies with various experimental systems of the regulation of specific mRNA levels.
Journal ArticleDOI
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
TL;DR: A modification of the Berk-Sharp technique is used to determine that the 5' termini of the mouse 15 S beta-globin precursor and the mature mRNA have identical map coordinates.