Book ChapterDOI
Structure of serum albumin.
Daniel C. Carter,Joseph X. Ho +1 more
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This chapter provides an insight of the findings of past significant papers with the current knowledge of the recently determined high resolution X-ray structure of serum albumin and suggests that AFP may have a higher affinity for some unknown ligands important for fetal development.Abstract:
Publisher Summary This chapter provides an insight of the findings of past significant papers with the current knowledge of the recently determined high resolution X-ray structure of serum albumin. The most outstanding property of albumin is its ability to bind reversibly an incredible variety of ligands. The sequences of all albumins are characterized by a unique arrangement of disulfide double loops that repeat as a series of triplets. Albumin belongs to a multigene family of proteins that includes α- fetoprotein (AFP) and vitamin D-binding protein (VDP), also known as G complement (Gc) protein. Although AFP is considered the fetal counterpart of albumin, its binding properties are distinct and it is suggested that AFP may have a higher affinity for some unknown ligands important for fetal development. Domain structure and the arrangement of the disulfides, the surface charge distribution, and the conformational flexibility of the albumin molecule are described. The nature of ligand binding, including small organics, long-chain fatty acids, and metals, to multiple sites on the albumin molecule is clearly depicted. The chapter concludes with the perceptive comments on future directions being taken to explore the structure and function of this fascinating protein.read more
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Journal ArticleDOI
Albumin as a drug carrier: design of prodrugs, drug conjugates and nanoparticles.
TL;DR: This review gives an account of the different drug delivery systems which make use of albumin as a drug carrier with a focus on those systems that have reached an advanced stage of preclinical evaluation or that have entered clinical trials.
Journal ArticleDOI
Crystal structure of human serum albumin at 2.5 A resolution.
TL;DR: A new triclinic crystal form of human serum albumin (HSA), derived either from pool plasma or from a Pichia pastoris expression system, was obtained from polyethylene glycol 4000 solution, and three-dimensional structures of pHSA and rHSA were determined.
Journal ArticleDOI
Structure and reactivity of water at biomaterial surfaces
TL;DR: It is found that solvent properties of water within the interphase separating a solid surface from bulk water solution vary with contacting surface chemistry, and this interphase can extend tens of nanometers from a water-contacting surface due to a propagation of differences in self association between vicinal water and bulk-phase water.
Journal ArticleDOI
Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites
TL;DR: The crystal structure of HSA complexed with five molecules of myristate at 2.5 Å resolution is determined and it is shown that fatty acid molecules bind in long, hydrophobic pockets capped by polar side chains, many of which are basic.
Journal ArticleDOI
Human serum albumin: from bench to bedside.
TL;DR: HSA is a valuable biomarker of many diseases, including cancer, rheumatoid arthritis, ischemia, post-menopausal obesity, severe acute graft-versus-host disease, and diseases that need monitoring of the glycemic control.
References
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Laser-excited Raman spectroscopy of biomolecules. VIII. Conformational study of bovine serum albumin.
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Interactions of the carboxyl group of oleic acid with bovine serum albumin: a 13C NMR study.
TL;DR: The results suggested that the anionic form of oleic acid is bound to BSA at physiological pH and that the multiplicity of NMR peaks for [1-13C]oleic acid resulted from, at least in part, different electrostatic and hydrogen bonding interactions between the oleoic acid carboxyl group and specific amino acid residues of BSA.
Journal ArticleDOI
Serum Albumin. II. Identification of More than One Albumin in Horse and Human Serum by Electrophoretic Mobility in Acid Solution
Journal ArticleDOI
The neutral transition and the environment of the sulfhydryl side chain of bovine plasma albumin.
Journal ArticleDOI
Alpha-feto-protein during development and in disease.
A Adinolfi,M Adinolfi,Lessof +2 more
TL;DR: The detection of high levels of AFP in amniotic fluid has proved to be of great value for the prenatal diagnosis of neural-tube defects and is now being assessed as a method of monitoring abnormal pregnancy.