Journal ArticleDOI
Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid
William I. Weis,Jerry H. Brown,Stephen Cusack,Stephen Cusack,James C. Paulson,John J. Skehel,Don C. Wiley +6 more
TLDR
The three-dimensional structures of influenza virus haemagglutinins complexed with cell receptor analogues show sialic acids bound to a pocket of conserved amino acids surrounded by antibody-binding sites, suggesting that antibodies neutralize virus infectivity by preventing virus-to-cell binding.Abstract:
The three-dimensional structures of influenza virus haemagglutinins complexed with cell receptor analogues show sialic acids bound to a pocket of conserved amino acids surrounded by antibody-binding sites. Sialic acid fills the conserved pocket, demonstrating that it is the influenza virus receptor. The proximity of the antibody-binding sites suggests that antibodies neutralize virus infectivity by preventing virus-to-cell binding. The structures suggest approaches to the design of anti-viral drugs that could block attachment of viruses to cells.read more
Citations
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Practical synthesis of d-[1-13C]mannose, l-[1-13C] and l-[6-13C]fucose
Ken-ichi Sato,Shoji Akai,Hiroki Youda,Masaru Kojima,Mayumi Sakuma,Shu-ichirou Inaba,Kyota Kurosawa +6 more
TL;DR: In this paper, a new method for the synthesis of [1-13 C]-labeled compounds via the corresponding olefin compounds, which are in turn derived from d -mannitol or l -arabinose by efficient introduction of 13 C, by the Wittig reaction using Ph 3 P 13 CH 3 I and n -BuLi.
Journal ArticleDOI
Antigenic variations of human influenza virus in Shiraz, Iran
Afagh Moattari,H. Ashrafi,M. R. Kadivar,M. T. Kheiri,M. Shahidi,M. Arabpour,Ali Mohammad Ghanbari +6 more
TL;DR: The H3N2 subtype strains prevalent during the 2005/7 influenza outbreak in southern Iran demonstrated a drastic antigenic variation and differed from A/California/7/2004 vaccine strain.
Journal ArticleDOI
The therapeutic potential of sialylated Fc domains of human IgG.
TL;DR: In this article, the authors describe the development of molecular scaffolds based on the crystallizable fragment (Fc) region of immunoglobulin (Ig) G that deliver high-avidity binding to innate immune receptors, including sialic acid-dependent receptors.
Alternative approaches in the preparation and growth of influenza B vaccine viruses
TL;DR: A large number of influenza B antigens are present in all human vaccines, and increases in yields have been observed with increasing levels of influenza A viruses.
Journal ArticleDOI
Chemoenzymatic synthesis and characterization of N-glycolylneuraminic acid-carrying sialoglycopolypeptides as effective inhibitors against equine influenza virus hemagglutination
Makoto Ogata,Ami Koizumi,Tadamune Otsubo,Kiyoshi Ikeda,Mao Sakamoto,Rena Aita,Tatsuya Kato,Enoch Y. Park,Takashi Yamanaka,Kazuya I.P.J. Hidari +9 more
TL;DR: The findings show the multivalent α2,3-linked Neu5Gc-ligands selectively inhibit hemagglutination mediated by both influenza viruses and display a strong inhibitory activity.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Areas, volumes, packing and protein structure.
TL;DR: This review is concerned with the packing of groups of atoms in proteins and with the area of solvent-protein interfaces.
Journal ArticleDOI
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
TL;DR: The haemagglutinin glycoprotein of influenza virus is a trimer comprising two structurally distinct regions: a triple-stranded coiled-coil of α-helices extends 76 Å from the membrane and a globular region of antiparallel β-sheet is positioned on top of this stem.
Journal ArticleDOI
Aromatic-aromatic interaction: a mechanism of protein structure stabilization
TL;DR: Analysis of neighboring aromatic groups in four biphenyl peptides or peptide analogs and 34 proteins reveals a specific aromatic-aromatic interaction that helps stabilize tertiary structure, and 20 percent stabilize quaternary structure.
Journal ArticleDOI
Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation
TL;DR: Four ‘antigenic sites’ on the three-dimensional structure of the influenza haemagglutinin are identified and at least one amino acid substitution in each site seems to be required for the production of new epidemic strains between 1968 and 1975.
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Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
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