Journal ArticleDOI
Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid
William I. Weis,Jerry H. Brown,Stephen Cusack,Stephen Cusack,James C. Paulson,John J. Skehel,Don C. Wiley +6 more
TLDR
The three-dimensional structures of influenza virus haemagglutinins complexed with cell receptor analogues show sialic acids bound to a pocket of conserved amino acids surrounded by antibody-binding sites, suggesting that antibodies neutralize virus infectivity by preventing virus-to-cell binding.Abstract:
The three-dimensional structures of influenza virus haemagglutinins complexed with cell receptor analogues show sialic acids bound to a pocket of conserved amino acids surrounded by antibody-binding sites. Sialic acid fills the conserved pocket, demonstrating that it is the influenza virus receptor. The proximity of the antibody-binding sites suggests that antibodies neutralize virus infectivity by preventing virus-to-cell binding. The structures suggest approaches to the design of anti-viral drugs that could block attachment of viruses to cells.read more
Citations
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Journal ArticleDOI
Continuous Production of Sialyllactose from Colominic Acid Using a Membrane Reactor
TL;DR: The effects of various reaction conditions on sialyllactose productivity were investigated, and it was found that the productivity was independent of the enzyme concentration and reaction temperature but dependent on the substrate and buffer concentrations and the hydraulic retention time (HRT).
Book ChapterDOI
Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination Therapies.
TL;DR: This chapter describes the following aspects of updated sialyl sugar chains as influenza A virus HA inhibitors (HAIs) and discusses about the use of HAI-based combinations that should be considered for future influenza therapy.
Book ChapterDOI
Strategies for discovering antiviral agents from natural products.
E. Rozhon,R. Albin,J. Schwartz +2 more
TL;DR: This chapter discusses the strategies for discovering antiviral agents from natural products and it is found that when developing assays to detect agents that specifically affect a given viral process, it is important to keep in mind the intimate relationship that exists between the host cell and pathogen.
Journal ArticleDOI
The application of pseudotypes to influenza pandemic preparedness
TL;DR: This review compares pseudotype-based assays with wild-type and virus-like particle virus assays and discusses the extent of pre-existing heterosubtypic immunity in populations.
Journal ArticleDOI
Antibodies against an α-bungarotoxin-binding peptide of the α-subunit of the acetylcholine receptor
TL;DR: Epitope mapping revealed that the antibodies are directed against residues 183–194 indicating this region is a major determinant of toxin binding, most likely conformationally constrained in the native receptor.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Areas, volumes, packing and protein structure.
TL;DR: This review is concerned with the packing of groups of atoms in proteins and with the area of solvent-protein interfaces.
Journal ArticleDOI
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
TL;DR: The haemagglutinin glycoprotein of influenza virus is a trimer comprising two structurally distinct regions: a triple-stranded coiled-coil of α-helices extends 76 Å from the membrane and a globular region of antiparallel β-sheet is positioned on top of this stem.
Journal ArticleDOI
Aromatic-aromatic interaction: a mechanism of protein structure stabilization
TL;DR: Analysis of neighboring aromatic groups in four biphenyl peptides or peptide analogs and 34 proteins reveals a specific aromatic-aromatic interaction that helps stabilize tertiary structure, and 20 percent stabilize quaternary structure.
Journal ArticleDOI
Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation
TL;DR: Four ‘antigenic sites’ on the three-dimensional structure of the influenza haemagglutinin are identified and at least one amino acid substitution in each site seems to be required for the production of new epidemic strains between 1968 and 1975.
Related Papers (5)
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
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