Superoxide dismutase (SOD) as a selection criterion for triticale grain yield under drought stress: a comprehensive study on genomics and expression profiling, bioinformatics, heritability, and phenotypic variability.
Armin Saed-Moucheshi,Fatemeh Sohrabi,Elham Fasihfar,Fatemeh Baniasadi,Mehrnaz Riasat,Ali Akbar Mozafari +5 more
TLDR
In this paper, a new programing based on SAS-language (IML) was introduced to estimate the genetic parameters rooted from combined ANOVA model (linear mixed model), which is capable of being used in any field study other than the current one.Abstract:
The main objectives of this study were to find the possible structural association between the activity of enzymatic antioxidants and the grain yield of triticale plants as well as identifying the genotypic variability which might be effective on this association. Accordingly, expression levels of superoxide dismutase (SOD) isozymes (Mn-SOD, Cu/Zn-SOD, and Fe-SOD) were appraised to distinguish any possible relationship between SOD expression and drought resistance of triticale. A novel analytical method for distinguishing elite genotypes based on measured features was proposed. Additionally, a new programing based on SAS-language (IML) was introduced to estimate the genetic parameters rooted from combined ANOVA model (linear mixed model), which is capable of being used in any field study other than the current one. Thirty genotypes of triticale were studied under normal and drought stress conditions during 6 years (three different locations). Accordingly, based on the results of genetic variability, heatmap analysis, biplot graph, and clustering technique, two genotypes with the highest genetic distance were selected to appraise the differential expression profiling of three SOD isozyme in shoot and root organs. Field experiments and bioinformatics results showed that superoxide dismutase (SOD) was the most influential antioxidant in resistance of triticale to drought stress; therefore, it could be used as an indirect selection index in early stages to distinguish resistant genotypes to drought stress. Additionally, Mn-SOD and Fe-SOD showed roughly similar expression levels for both genotypes under drought stress. However, Cu/Zn-SOD expression level was higher in root and shoot of the tolerant genotype than the susceptible genotype. Heatmap analysis that is applied for the first time to screen suitable genotypes, showed to be highly capable of distinguishing elite genotypes and pointing out the proper features for selection criteria. Bioinformatics results indicated that SOD is more important than other enzymatic antioxidant for being considered as selection criteria or candidate gene for transgenic purposes. Based on expressional results, Mn-SOD announced as a general isozyme that is probably highly expressed in most of the species, while, Cu/Zn-SOD was introduced as a genotype specific isozyme that is likely more expressed in tolerant genotypesread more
Citations
More filters
Journal ArticleDOI
Screening barley varieties tolerant to drought stress based on tolerant indices
Armin Saed-Moucheshi,Mohammad Pessarakli,Ali Akbar Mozafari,Fatemeh Sohrabi,Mahsa Moradi,Fatemeh Barzegar Marvasti +5 more
TL;DR: The overall results of biplot showed that varieties Danesiah, Eram, and Yoosef, which had higher grain yield than average for both conditions, were suitable varieties in order to be screened for both normal and drought stress conditions.
Journal ArticleDOI
Insilco identification and characterization of superoxide dismutase gene family in Brassica rapa.
Asif M. Iqbal Qureshi,Mehraj uddin Sofi,Niyaz A. Dar,Mudasir H. Khan,S. S. Mahdi,Z.A. Dar .,Shabir A. Bangroo,Hamed A. El-Serehy,Daniel Ingo Hefft,Simona Mariana Popescu +9 more
TL;DR: In this paper, a genome wide study of SOD gene family was conducted to identify BrSOD genes, their domain-based organization, gene structure analysis, phylogenetic analysis, intron-exon structure of genes and expression analysis.
Journal ArticleDOI
Agronomical and Physiological Responses of Faba Bean Genotypes to Salt Stress
TL;DR: In this paper , the authors evaluated faba bean genotypes in response to saline stress and identified those that were tolerant and determined the influential ratio of each yield component on seed yield under both conditions.
Journal ArticleDOI
Salicylic Acid Improves Antioxidant Defense System and Photosynthetic Performance in Aristotelia chilensis Plants Subjected to Moderate Drought Stress
Jorge González-Villagra,Marjorie Reyes-Díaz,Ricardo Tighe-Neira,Claudio Inostroza-Blancheteau,Ana Luengo Escobar,León A. Bravo +5 more
TL;DR: In this paper , the role of Salicylic acid (SA) application on enzymatic and non-enzymatic antioxidants, photosynthetic performance, and plant growth in A. chilensis plants was investigated.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Rapid determination of free proline for water-stress studies
TL;DR: In this article, a simple colorimetric determination of proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material was presented.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.
Journal ArticleDOI
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Yoshiyuki Nakano,Kozi Asada +1 more
TL;DR: Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.
Book ChapterDOI
The assay of catalases and peroxidases.
Britton Chance,A.C. Maehly +1 more
TL;DR: Two methods are described for the catalase assay by disappearance of peroxide are: ultraviolet spectrophotometry and permanganate titration and indirect measurements of the decrease of light absorption caused by the decomposition of hydrogen peroxide byCatalase.