Journal ArticleDOI
Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells.
Yechun Wang,Oliver Yu +1 more
TLDR
This work demonstrated the synthetic scaffolds can be used for the optimization of engineered metabolic pathway and observed a 5.0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein.About:
This article is published in Journal of Biotechnology.The article was published on 2012-01-01. It has received 133 citations till now. The article focuses on the topics: Resveratrol.read more
Citations
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Journal ArticleDOI
Substrate channelling as an approach to cascade reactions.
Ian Wheeldon,Shelley D. Minteer,Scott Banta,Scott Calabrese Barton,Plamen Atanassov,Matthew S. Sigman +5 more
TL;DR: The incorporation of substrate channelling into synthetic cascades is a rapidly developing concept, and recent examples of the fabrication of cascades with controlled diffusion and flux of intermediates are presented.
Journal ArticleDOI
De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae
Frank Koopman,Jules Beekwilder,Barbara Crimi,Adèle van Houwelingen,Robert Hall,Dirk Bosch,Antonius J. A. van Maris,Jack T. Pronk,Jean-Marc Daran +8 more
TL;DR: The results reported in this study demonstrate that S. cerevisiae is capable of de novo production of naringenin by coexpressing the naringENin production genes from A. thaliana and optimization of the flux towards the naredenin pathway.
Journal ArticleDOI
Metabolic engineering of muconic acid production in Saccharomyces cerevisiae.
TL;DR: This work collectively demonstrates that yeast has the potential to be a platform for the bioproduction of muconic acid and suggests an area that is ripe for future metabolic engineering efforts.
Journal ArticleDOI
De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae
Mingji Li,Kanchana Rueksomtawin Kildegaard,Yun Chen,Angelica Rodriguez,Irina Borodina,Jens Nielsen,Jens Nielsen +6 more
TL;DR: The yeast Saccharomyces cerevisiae is engineered to produce resveratrol directly from glucose or ethanol via tyrosine intermediate through the biosynthetic pathway, and the supply of the precursor malonyl-CoA is improved by over-expressing a post-translational de-regulated version of the acetyl- CoA carboxylase encoding gene ACC1.
Journal ArticleDOI
Production of natural products through metabolic engineering of Saccharomyces cerevisiae.
TL;DR: Recent advances in reconstruction of the biosynthetic pathways of various high-value products in the yeast Saccharomyces cerevisiae, a commonly used industrial microorganism are discussed.
References
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Journal ArticleDOI
Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan
Konrad T. Howitz,Kevin J. Bitterman,Haim Y. Cohen,Dudley W. Lamming,Siva Lavu,Jason G. Wood,Robert E. Zipkin,Phuong Chung,Anne Kisielewski,Li-Li Zhang,Brandy Scherer,David A. Sinclair +11 more
TL;DR: The potent activator resveratrol, a polyphenol found in red wine, lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD+, and increases cell survival by stimulating Sirt1-dependent deacetylation of p53.
Journal ArticleDOI
Synthetic protein scaffolds provide modular control over metabolic flux
John E. Dueber,Gabriel C. Wu,G Reza Malmirchegini,G Reza Malmirchegini,Tae Seok Moon,Christopher J. Petzold,Christopher J. Petzold,Adeeti Ullal,Kristala L. J. Prather,Jay D. Keasling +9 more
TL;DR: In this article, synthetic protein scaffolds bearing interaction domains from metazoan signaling proteins were used to spatially recruit metabolic enzymes in a designable manner, and the modularity of these domains enabled them to optimize the stoichiometry of three mevalonate biosynthetic enzymes recruited to a synthetic complex.
Journal ArticleDOI
Resveratrol Prolongs Lifespan and Retards the Onset of Age-Related Markers in a Short-Lived Vertebrate
Dario Riccardo Valenzano,Eva Terzibasi,Tyrone Genade,Antonino Cattaneo,Luciano Domenici,Alessandro Cellerino +5 more
TL;DR: It is demonstrated that food supplementation with resveratrol prolongs lifespan and retards the expression of age-dependent traits in a short-lived vertebrate.
Book ChapterDOI
Yeast expression of animal and plant p450s in optimized redox environments
TL;DR: Yeast Saccharomyces cerevisiae offers a low-cost and efficient way to express heterologous P450s and multiple genomic modifications yeast featuring redox environment optimized for P450 functions was developed.
Journal ArticleDOI
Cloning, Yeast Expression, and Characterization of the Coupling of Two Distantly Related Arabidopsis thalianaNADPH-Cytochrome P450 Reductases with P450 CYP73A5
TL;DR: Biochemical characterization of the Arabidopsis ATR1/CYP73A5 and ATR2-1/Yeast systems demonstrates that the two distantly relatedArabidopsis reductases similarly support the first oxidative step of the phenylpropanoid general pathway.