T cell-dependent mast cell degranulation and release of serotonin in murine delayed-type hypersensitivity.
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Light microscopic radioautographs from animals treated with [3H]5-HT indicated that local mast cells released 5-HT between 6 and 18 h during the evolution of DTH, which indicated that mast cell degranulation and 5- HT release in murine DTH were probably T cell dependent.Abstract:
We have previously suggested that the release of serotonin (5-hydroxytryptamine) (5-HT) by local tissue mast cells is required for the elicitation of delayed-type hypersensitivity (DTH) in mice. In the current study, light microscopic radioautographs from animals treated with [3H]5-HT indicated that local mast cells released 5-HT between 6 and 18 h during the evolution of DTH. Ultrastructural examination of mast cells revealed surface activation, indicated by extension of surface filopodia, and degranulation by fusion and exocytosis. Light and electron microscopic studies of the endothelium of postcapillary venules at sites of DTH revealed the development of gaps between adjacent cells. The development of gaps permitted extravasation of tracers that was abolished by depletion or antagonism of 5-HT. Thus mast cells degranulated and released 5-HT in DTH, and this 5-HT acted on local vessels. Recipients of nonadherent, non-immunoglobulin-bearing sensitized lymphocytes also demonstrated similar mast cell degranulation and the formation of endothelial gaps. This indicated that mast cell degranulation and 5-HT release in murine DTH were probably T cell dependent.read more
Citations
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Acute stress increases permeability of the blood-brain-barrier through activation of brain mast cells.
Pamela Esposito,Daniela Gheorghe,Kristiana Kandere,Xinzhu Pang,Raymond J. Connolly,Stanley Jacobson,Theoharis C. Theoharides +6 more
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Mast cells and inflammation.
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Differential release of serotonin and histamine from mast cells
TL;DR: The ability to secrete differentially expands the physiological potential of the mast cell, and suggests that release of serotonin may not always indicate mast cell secretion via exocytosis of secretory granules.
References
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STUDIES ON INFLAMMATION: I. The Effect of Histamine and Serotonin on Vascular Permeability: An Electron Microscopic Study
Guido Majno,G. E. Palade +1 more
TL;DR: The mechanism, whereby histamine and serotonin increase the permeability of blood vessels, was studied in the rat by means of the electron microscope, and electron microscopic findings suggested that the endothelial cells become partially disconnected along the intercellular junctions.
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Augmentation of delayed-type hypersensitivity by doses of cyclophosphamide which do not affect antibody responses.
TL;DR: The results suggest that antibody feedback is not the sole regulator of delayed reactions; the possibility that suppressor T cells may also be involved is discussed.
Journal ArticleDOI
Influence of dose and route of antigen injection on the immunological induction of T cells.
TL;DR: DTH fails to reappear in respose to secondary stimulation except in splenectomized mice in whom the development of DTH is not suppressed, even by massive doses of SRBC, and blocking of induction and expression may depend, therefore, on different mechanisms.
Journal ArticleDOI
Differential release of serotonin and histamine from mast cells
TL;DR: The ability to secrete differentially expands the physiological potential of the mast cell, and suggests that release of serotonin may not always indicate mast cell secretion via exocytosis of secretory granules.