Journal ArticleDOI
Taq polymerase contains bacterial DNA of unknown origin.
Kenneth H. Rand,Herbert J. Houck +1 more
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The polymerase chain reaction (PCR) was carried out with the highly conserved E. coli ribosomal RNA gene sequences 1376-1395 and 1521-1540, finding a 165 bp fragment that is likely other biological products are similarly contaminated.About:
This article is published in Molecular and Cellular Probes.The article was published on 1990-12-01. It has received 122 citations till now. The article focuses on the topics: Taq polymerase & Hot start PCR.read more
Citations
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Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Susannah J. Salter,Michael J. Cox,Elena M. Turek,Szymon T. Calus,William O.C.M. Cookson,Miriam F. Moffatt,Paul Turner,Paul Turner,Julian Parkhill,Nicholas J. Loman,Alan W. Walker,Alan W. Walker +11 more
TL;DR: It is demonstrated that contaminating DNA is ubiquitous in commonly used DNA extraction kits and other laboratory reagents, varies greatly in composition between different kits and kit batches, and that this contamination critically impacts results obtained from samples containing a low microbial biomass.
Journal ArticleDOI
Determination of microbial diversity in environmental samples: pitfalls of PCR‐based rRNA analysis
TL;DR: Specific aspects of sample collection, cell lysis, nucleic acid extraction, PCR amplification, separation of amplified DNA, application of nucleic probes and data analysis are covered.
Journal ArticleDOI
Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data.
Nicole M Davis,Diana M. Proctor,Diana M. Proctor,Susan Holmes,David A. Relman,David A. Relman,Benjamin J. Callahan +6 more
TL;DR: The application of decontam to two recently published datasets corroborated and extended their conclusions that little evidence existed for an indigenous placenta microbiome and that some low-frequency taxa seemingly associated with preterm birth were contaminants.
Journal ArticleDOI
Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates.
D. N. Fredericks,David A. Relman +1 more
TL;DR: A set of molecular guidelines for establishing disease causation with sequence-based technology is proposed, and the importance of the scientific concordance of evidence in supporting causal associations is emphasized.
Posted ContentDOI
Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data
TL;DR: The application of decontam to two recently published datasets corroborated and extended their conclusions that little evidence existed for an indigenous placenta microbiome, and that some low-frequency taxa seemingly associated with preterm birth were run-specific contaminants.
References
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Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
TL;DR: The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.
Journal ArticleDOI
Physical map of the seven ribosomal RNA genes of Escherichia coli.
TL;DR: The results suggest that phage lambda daroE152 carries a "hybrid" rRNA gene which was probably formed by recombination between two different chromosomal rRNA genes.
Journal ArticleDOI
A common origin of rickettsiae and certain plant pathogens
TL;DR: On the basis of ribosomal RNA sequence comparisons, the rickettsia Rochalimaea quintana has been found to be a member of subgroup 2 of the alpha subdivision of the so-called purple bacteria, which is one of about ten major eubacterial divisions.