Journal ArticleDOI
Terpene biosynthesis: formation of nerol, geraniol, and other prenols by an enzyme system from Pinus radiata seedlings.
TLDR
A soluble enzyme system obtained from Pinus radiata seedlings incorporates 6–7% of radioactivity from DL-2-14C mevalonic acid into isopentenol, dimethyl allyl alcohol, nerol, and geraniol, suggesting that neryl pyroph phosphate and not geranyl pyrophosphate may be the precursor of cyclic monoterpenes.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1969-01-01. It has received 39 citations till now. The article focuses on the topics: Nerol & Geranyl pyrophosphate.read more
Citations
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Book ChapterDOI
Control of Isoprenoid Biosynthesis in Higher Plants
TL;DR: This chapter considers the knowledge of the control of isoprenoid biosynthesis in higher plants, particularly with respect to the properties and subcellular locations of the enzymes involved.
Book ChapterDOI
The Biochemistry of Plant Sterols
TL;DR: The isolation and identification of a particular compound falls into the general realm of structural elucidation, which can become complicated, especially for an unusual sterol, however, for the common dominant structures, advances in chromatography and spectroscopy allow a simple procedure.
Journal ArticleDOI
Biosynthesis of monoterpenes: hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from sage (Salvia officinalis).
Rodney Croteau,Frank Karp +1 more
TL;DR: Soluble enzyme preparations from sage leaves catalyze the hydrolysis of (+)-bornyl pyrophosphate to (+)-borneol, which is an essential step in the biosynthesis of the cyclic monoterpene (+)-camphor in this tissue, and each activity was distinctly different with regard to molecular weight, pH optimum, response to inhibitors, and substrate specificity.
Journal ArticleDOI
Biosynthesis of monoterpene hydrocarbons from [1-3H]neryl pyrophosphate and [1-3H]geranyl pyrophosphate by soluble enzymes from Citrus limonum
TL;DR: A soluble enzyme preparation from the flavedo of Citrus limonum transforms neryl and geranyl pyrophosphate into β-pinene, sabinene, α-pinenes, and limonene, and the addition of reagents containing sulfhydryl groups is essential for optimal activity.
Journal ArticleDOI
Squalene Synthetase: III. MECHANISM OF THE REACTION
TL;DR: Initial velocity kinetics and product inhibition studies for the reduction of presqualene pyrophosphate to squalene have shown that this reaction is sequential ordered, and it is evident that a sulfhydryl group or other nucleophile is required for enzyme activity.
References
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Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.