Journal ArticleDOI
The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200.
Hui Peng,Guogan Wu,Weilan Shao +2 more
TLDR
A bifunctional aldehyde/alcohol dehydrogenase gene (adhE) from Thermoanaerobacter ethanolicus JW200 was identified and cloned and it was shown that AdhE affected ethanol production.About:
This article is published in Anaerobe.The article was published on 2008-04-01. It has received 53 citations till now. The article focuses on the topics: Thermoanaerobacter ethanolicus & Alcohol dehydrogenase.read more
Citations
More filters
Journal ArticleDOI
Thermophilic ethanologenesis: future prospects for second-generation bioethanol production
TL;DR: This review highlights the milestones in the development of metabolically engineered production strains that possess a high degree of catabolic versatility and are homoethanologenic, with particular focus on theDevelopment of reliable methods for cell competency, gene deletion or upregulation.
Journal ArticleDOI
Metabolic engineering of Clostridium autoethanogenum for selective alcohol production
TL;DR: It is demonstrated that AOR is critical to ethanol formation in acetogens and inactivation of AdhE led to consistently enhanced autotrophic ethanol production (up to 180%).
Journal ArticleDOI
Bioenergetic constraints for conversion of syngas to biofuels in acetogenic bacteria
Johannes Bertsch,Volker Müller +1 more
TL;DR: This review discusses the bioenergetic constraints for the conversion of syngas to different biofuels and focuses on Acetobacterium woodii, which is the best understood acetogen in terms of energy conservation.
Journal ArticleDOI
Ethanol production by engineered thermophiles
TL;DR: It is found that there is not an example where a thermophilic ethanol production pathway has been transferred to a non-ethanol-producing organism to produce ethanol at high yield.
Journal ArticleDOI
State of the art review of biofuels production from lignocellulose by thermophilic bacteria
Yujia Jiang,Fengxue Xin,Jiasheng Lu,Weiliang Dong,Wenming Zhang,Min Zhang,Hao Wu,Jiangfeng Ma,Min Jiang +8 more
TL;DR: This review comprehensively summarizes the state of the art development of biofuels production by reported thermophilic microorganisms, and also concludes strategies to improve bio Fuels production including the metabolic pathways construction, co-culturing systems and biofuelels tolerance.
References
More filters
Journal ArticleDOI
Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new, extreme thermophilic, anaerobic bacterium
Jürgen Wiegel,Lars G. Ljungdahl +1 more
TL;DR: Two strains, JW 200 and JW 201, of an extreme thermophilic, non-spore-forming anaerobic bacterium were isolated from alkaline and slightly acidic hot springs located in Yellowstone National Park and the name Thermoanaerobacter ethanolicus gen. nov.
Journal ArticleDOI
Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824
Lisa Fontaine,Isabelle Meynial-Salles,Laurence Girbal,Xinghong Yang,Christian Croux,Philippe Soucaille,Philippe Soucaille +6 more
TL;DR: The adhE2 gene of Clostridium acetobutylicum ATCC 824, coding for an aldehyde/alcohol dehydrogenase (AADH), was characterized from molecular and biochemical points of view and is the first example of a bacterium with two AADHs.
Journal ArticleDOI
Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE
TL;DR: The identity of PFL‐deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli was revealed and a mutant lacking deactivase was shown to require exogenous electron acceptors for anaecrobic growth with glucose.
Journal ArticleDOI
Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase.
D S Burdette,J. G. Zeikus +1 more
TL;DR: The results suggest that the 2 degrees Adh primarily functions in ethanol production from acetyl-CoA and acetaldehyde, whereas the 1 degree Adh functions inanol consumption for nicotinamide-cofactor recycling.
Journal ArticleDOI
Evolution of the adhE Gene Product ofEscherichia coli from a Functional Reductase to a Dehydrogenase GENETIC AND BIOCHEMICAL STUDIES OF THE MUTANT PROTEINS
Jorge Membrillo-Hernández,Pedro Echave,Elisa Cabiscol,Jordi Tamarit,Joaquim Ros,Edmund C. C. Lin +5 more
TL;DR: It appears that when AdhE catalyzes the two sequential reactions in the counter-physiological direction, acetaldehyde dehydrogenation is the rate-limiting step.