The anaerobic bacterial flora of the mouse cecum.
James H. Gordon,René J. Dubos +1 more
TLDR
Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerilic bacteria from the cecum of SPF mice.Abstract:
Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The commonest morphologic types of bacteria in the mouse cecum were tapered rods. These characteristically gave "speckled" colonies on agar plates. Those identified were species of the genera Fusobacterium, Eubacterium, and Clostridium. Several of the morphologic types seen with phase microscopy still could not be cultivated. The implications of these findings for the study of the intestinal flora are discussed.read more
Citations
More filters
Journal ArticleDOI
Gut Microbiota in Health and Disease
TL;DR: The advances in modeling and analysis of gut microbiota will further the authors' knowledge of their role in health and disease, allowing customization of existing and future therapeutic and prophylactic modalities.
Journal ArticleDOI
How host-microbial interactions shape the nutrient environment of the mammalian intestine
TL;DR: This paper showed that Bacteroides thetaiotaomicron, a prominent and genetically manipulatable component of the normal human and mouse gut, modifies many aspects of intestinal cellular differentiation/gene expression to benefit both host and microbe.
Journal ArticleDOI
Survival of human dental plaque flora in various transport media.
Salam A. Syed,W.J. Loesche +1 more
TL;DR: It is suggested that RTF is a satisfactory medium for the transport of oral bacteria present in the samples and VMG II showed a higher recovery of organisms from these specimens with an increase in the storage period, suggesting multiplication of the plaque flora.
Journal ArticleDOI
Phylogeny of the Defined Murine Microbiota: Altered Schaedler Flora
Floyd E. Dewhirst,Chih-Ching Chien,Bruce J. Paster,Rebecca L. Ericson,Roger P. Orcutt,David B. Schauer,James G. Fox +6 more
TL;DR: The 16S rRNA sequence information determined in the present study should allow rapid identification of ASF strains and should permit detailed analysis of the interactions of AsF organisms during development of intestinal disease in mice that are coinfected with a variety of pathogenic microorganisms.
Journal ArticleDOI
The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota
Ilias Lagkouvardos,Rüdiger Pukall,Birte Abt,Bärbel U. Foesel,Jan P. Meier-Kolthoff,Neeraj Kumar,Anne Gøther Bresciani,Inés Martínez,Sarah Just,Caroline Ziegler,Sandrine Brugiroux,Debora Garzetti,Mareike Wenning,Thi Phuong Nam Bui,Jun Wang,Floor Hugenholtz,Caroline M. Plugge,Daniel A. Peterson,Mathias W. Hornef,John F. Baines,Hauke Smidt,Jens Walter,Karsten Kristiansen,Henrik Nielsen,Dirk Haller,Jörg Overmann,Bärbel Stecher,Thomas Clavel +27 more
TL;DR: Genomic analyses showed that certain species are specific to the mouse intestine and that a minimal consortium of 18 strains covered 50–75% of the known functional potential of metagenomes, which will sustain future research on microbiota–host interactions in health and disease.
References
More filters
Journal ArticleDOI
The gastrointestinal epithelium and its autochthonous bacterial flora
TL;DR: The bacteriological and histological findings described here constitute further evidence for the hypothesis that symbiotic associations exist between microorganisms and animals, and that a very large percentage of the bacteria in the gastrointestinal tract constitutes a true autochthonous flora.
Journal ArticleDOI
The development of the bacterial flora in the gastrointestinal tract of mice
TL;DR: It has been found that the lactobacilli and anaerobic streptococci become established immediately after birth and persist in large numbers, not only in the large intestine but also in the stomach and in the small intestine, while other bacterial species become established at different periods of time after birth, exhibit characteristic anatomic localizations, and greatly fluctuate in numbers.
Journal ArticleDOI
Alterations in the mouse cecum and its flora produced by antibacterial drugs
Dwayne C. Savage,René J. Dubos +1 more
TL;DR: Observations support the hypothesis that certain elements of the intestinal microflora play an essential role in maintaining the integrity of the water-transport mechanism in the intestinal epithelium.
Journal ArticleDOI
Techniques for routine culture of fastidious anaerobes
TL;DR: Experimental equipment and techniques which have been developed for the production of highly reduced media and for the handling and inoculation of large numbers of fastidiously anaerobic bacteria are described.
Journal ArticleDOI
Anaerobic Bacteria on the Mucosal Epithelium of the Murine Large Bowel
TL;DR: Anaerobic bacteria can be detected at population levels of 10(11) organisms per g of cecum or colon in adult mice from four different colonies widely spaced in the United States, and one anaerobic spiral bacterium was isolated in pure culture.