The Arabidopsis SLEEPY1 Gene Encodes a Putative F-Box Subunit of an SCF E3 Ubiquitin Ligase
Karen M. McGinnis,Stephen G. Thomas,Jonathan D. Soule,Lucia C. Strader,Janice M. Zale,Tai-ping Sun,Camille M. Steber +6 more
TLDR
The predicted SLY1 amino acid sequence is highly conserved among plants, indicating a key role in GA response, and suggests SCFSLY1-targeted degradation of RGA through the 26S proteasome pathway.Abstract:
The Arabidopsis SLY1 (SLEEPY1) gene positively regulates gibberellin (GA) signaling. Positional cloning of SLY1 revealed that it encodes a putative F-box protein. This result suggests that SLY1 is the F-box subunit of an SCF E3 ubiquitin ligase that regulates GA responses. The DELLA domain protein RGA (repressor of ga1-3) is a repressor of GA response that appears to undergo GA-stimulated protein degradation. RGA is a potential substrate of SLY1, because sly1 mutations cause a significant increase in RGA protein accumulation even after GA treatment. This result suggests SCFSLY1-targeted degradation of RGA through the 26S proteasome pathway. Further support for this model is provided by the observation that an rga null allele partially suppresses the sly1-10 mutant phenotype. The predicted SLY1 amino acid sequence is highly conserved among plants, indicating a key role in GA response.read more
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Auxin: regulation, action, and interaction.
Andrew Woodward,Bonnie Bartel +1 more
TL;DR: Nearly six decades after the structural elucidation of IAA, many aspects of auxin metabolism, transport and signalling are well established; however, more than a few fundamental questions and innumerable details remain unresolved.
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The ubiquitin 26S proteasome proteolytic pathway
TL;DR: Current understanding of the Ub/26S proteasome pathway in plants is described at the biochemical, genomic, and genetic levels, using Arabidopsis thaliana as the model, showing that this pathway is one of the most elaborate regulatory mechanisms in plants.
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Molecular Aspects of Seed Dormancy
TL;DR: The net result is a slightly heterogeneous response, thereby providing more temporal options for successful germination.
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A molecular framework for light and gibberellin control of cell elongation
Miguel de Lucas,Jean-Michel Davière,Mariana Rodríguez-Falcón,Mariela Pontin,Juan Manuel Iglesias-Pedraz,Séverine Lorrain,Christian Fankhauser,Miguel A. Blázquez,Elena Titarenko,Salomé Prat +9 more
TL;DR: Destabilization of this factor by phyB, together with its inactivation by DELLAs, constitutes a protein interaction framework that explains how plants integrate both light and GA signals to optimize growth and development in response to changing environments.
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Growth-defense tradeoffs in plants: a balancing act to optimize fitness.
TL;DR: Evidence supporting the growth-defense tradeoff concept is addressed, as well as known interactions between defense signaling and growth signaling, which should provide a foundation for the development of breeding strategies to maximize crop yield to meet rising global food and biofuel demands.
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