Journal ArticleDOI
The assay of thyroxine binding globulin in human serum using nephelometry.
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TLDR
A nephelometric assay for the determination of thyroxine binding globulin concentration in human serum is described that has similar sensitivity to other non-radioactive protein assay, is rapid and highly reproducible and the problem of high background interference is circumvented.About:
This article is published in Clinica Chimica Acta.The article was published on 1980-04-11. It has received 4 citations till now. The article focuses on the topics: Thyroxine-binding globulin & Nephelometry.read more
Citations
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Journal ArticleDOI
Enzyme immunoassay of thyroxin-binding globulin.
TL;DR: A new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum using a TBG-beta-D-galactosidase complex prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester was described.
Journal ArticleDOI
Targeted sequencing panels in Italian ALS patients support different etiologies in the ALS/FTD continuum
Anna Bartoletti-Stella,Veria Vacchiano,Silvia de Pasqua,Giacomo Mengozzi,Dario de Biase,Ilaria Bartolomei,Patrizia Avoni,Giovanni Rizzo,Piero Parchi,Vincenzo Donadio,Adriano Chiò,Annalisa Pession,Federico Oppi,Fabrizio Salvi,Rocco Liguori,Sabina Capellari,BoReALS +16 more
TL;DR: In this paper, the authors analyzed ALS and ALS/FTD associated genes by direct sequencing or next-generation sequencing multigene panels in ALS patients and found clustering of variants in ALS major genes in patients with a family history for “pure” ALS.
Journal ArticleDOI
Time-resolved immunofluorometric assay for thyroxine-binding globulin in serum
TL;DR: A new "sandwich"-type immunofluorometric assay for thyroxine-binding globulin (TBG) in serum that involves a solid-phase monoclonal antibody immobilised in white microtiter wells, and a soluble biotinylated monoconal antibody that reacts with the captured TBG molecules.
Book ChapterDOI
Targeting Age-Related Neurodegenerative Diseases by AAV-Mediated Gene Therapy.
TL;DR: In this article, the advantages of AAV-based gene therapies are discussed with emphasis on efforts of developing novel capsids with superior therapeutic efficacy, and the results of clinical trials on AD, PD, and ALS are summarized.
References
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Journal ArticleDOI
Immunochemical quantitation of antigens by single radial immunodiffusion
TL;DR: By standardizing the technical conditions of the experiment it is possible to use this principle for the immunochemical determination of antigens, and the lower limit of the method was found to correspond to 0·0025 μg of antigen, and to an antigen concentrations of 1·25 μg per ml.
Journal ArticleDOI
Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies
TL;DR: The method utilizes the difference between the rate of electrophoretic migration of proteins and of their antibody complexes in agarose gel to perform quantitative analysis of proteins with a charge differing from that of the bulk of the immunoglobulins.
Journal ArticleDOI
Use of laser nephelometry in the measurement of serum proteins.
TL;DR: A laser nephelometer/reagent system for measuring serum proteins by the use of forward light scatter, electronic blank subtraction, laser light, and electronic signal selection provides a rapid, accurate, precise, and objective way to measure immunoglobulins and C3.
Journal ArticleDOI
Manual Nephelometric Methods for Immunochemical Determination of Immunoglobulins IgG, IgA, and IgM in Human Serum
L. M. Killingsworth,John Savory +1 more
TL;DR: Manual procedures for determining immunoglobulins IgG, IgA, and IgM in human serum correlated excellently with results of either radial immunodiffusion or an automated immunochemical procedure.
Journal ArticleDOI
Concentration of thyroxine-binding globulin: value of direct assay.
TL;DR: Comparison of a direct measurement of TBG concentration with a widely used indirect method showed that the indirect method was inaccurate when TBG concentrations were high, which will result in an increase in the derived free thyroxine index (FTI), so that euthyroid patients with a raisedTBG concentration may be at risk of being labelled thyrotoxic.
Related Papers (5)
Solid-phase radioimmunoassay of thyroxine in untreated serum
The use of quantitative immunoelectrophoresis to investigate thyroxine-binding human serum proteins.
T. Freeman,J.D. Pearson +1 more
[Measurement of free thyroxine in serum with radioimmunoassay (author's transl)].
T Mitsuma,T Nogimori +1 more