Journal ArticleDOI
The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy
TLDR
It is concluded that thisospholipid fraction (which contains the majority of the choline-containing phospholipids and some phosphatidylethanolamine) forms the outer monolayer of the membrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1973-10-11. It has received 1022 citations till now. The article focuses on the topics: Phosphatidylcholine & Sphingomyelin.read more
Citations
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The human ATP-binding cassette (ABC) transporter superfamily
TL;DR: The ATP-binding cassette (ABC) transporters are essential for many processes in the cell and mutations in these genes cause or contribute to several human genetic disorders including cystic fibrosis, neurological disease, retinal degeneration, cholesterol and bile transport defects, anemia, and drug response.
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Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides.
TL;DR: This review, which is focused on the different stages of membrane permeation induced by representatives of amphipathic alpha-helical antimicrobial and cell non-selective lytic peptides distinguishes between the 'carpet' mechanism, which holds for antimicrobial peptides versus the 'barrel-stave' mechanisms, which hold for cellnon- selective lytics peptides.
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Mode of action of membrane active antimicrobial peptides.
TL;DR: Although many studies support that bacterial membrane damage is a lethal event for bacteria, other studies point to a multihit mechanism in which the peptide binds to several targets in the cytoplasmic region of the bacteria.
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Biological Membranes as Bilayer Couples. A Molecular Mechanism of Drug-Erythrocyte Interactions
Michael P. Sheetz,S. J. Singer +1 more
TL;DR: It is proposed that membranes whose proteins and polar lipids are distributed asymmetrically in the two halves of the membrane bilayer can act as bilayer couples, i.e., theTwo halves can respond differently to a perturbation.
Journal ArticleDOI
Lipid asymmetry in membranes
TL;DR: Three sections of this chapter describing various approaches to study membrane lipid asymmetry are written by three different groups.
References
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Journal ArticleDOI
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
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A rapid and sensitive sub-micro phosphorus determination
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Asymmetrical lipid bilayer structure for biological membranes.
TL;DR: It is shown that the structure of the matrix of cellular membranes is a bimolecular leaflet of phospholipid molecules in which the polar heads reside on the outer surfaces of the bilayer, in contact with the aqueous environment.
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MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
TL;DR: The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin, indicating that fracture does not show membrane-surface detail, and results were consistent with the concept that membranes split during the fracture process of Freeze-etching.
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Localization of red cell membrane constituents.
TL;DR: A new concept for the haemolytic behaviour of mixtures ofospholipases is proposed, based upon a highly asymmetric distribution of the phospholipids in a bimolecular leaflet, and taking into account some aspects of the action of phospholIPases towards red cell ghosts.