The Calcineurin B-Like Calcium Sensors CBL1 and CBL9 Together with Their Interacting Protein Kinase CIPK26 Regulate the Arabidopsis NADPH Oxidase RBOHF
Maria M. Drerup,Kathrin Schlücking,Kenji Hashimoto,Prabha Manishankar,Leonie Steinhorst,Kazuyuki Kuchitsu,Jörg Kudla +6 more
TLDR
A direct interconnection between CBL-CIPK-mediated Ca(2+) signaling and ROS signaling in plants is identified and evidence for a synergistic activation of the NADPH oxidase RBOHF is provided by directCa(2+)-binding to its EF-hands and Ca( 2+)-induced phosphorylation by CBL1/9-C IPK26 complexes.About:
This article is published in Molecular Plant.The article was published on 2013-03-01 and is currently open access. It has received 327 citations till now. The article focuses on the topics: NADPH oxidase & Signal transduction.read more
Citations
More filters
Journal ArticleDOI
Ca2+-dependent phosphoregulation of the plasma membrane Ca2+-ATPase ACA8 modulates stimulus-induced calcium signatures.
Alex Costa,Laura Luoni,Claudia Adriana Marrano,Kenji Hashimoto,Philipp Köster,Sonia Giacometti,Maria Ida De Michelis,Jörg Kudla,Maria Cristina Bonza +8 more
TL;DR: The plasma membrane Ca2-ATPase ACA8 is a novel target of Ca2+-dependent CIPK–CBL complexes which tunes the pump activity affecting a stimulus-induced cytosolic Ca 2+ transient in planta.
Journal ArticleDOI
The CBL-CIPK Pathway in Plant Response to Stress Signals
TL;DR: This review describes the conserved structure of CBLs and CIPKs, characterize the features of classification and localization, draw conclusions about the currently known mechanisms, and summarizes the physiological functions of the CBL–CIPK network.
Journal ArticleDOI
A New β-Estradiol-Inducible Vector Set that Facilitates Easy Construction and Efficient Expression of Transgenes Reveals CBL3-Dependent Cytoplasm to Tonoplast Translocation of CIPK5
Kathrin Schlücking,Kai H. Edel,Philipp Köster,Maria M. Drerup,Christian Eckert,Leonie Steinhorst,Rainer Waadt,Rainer Waadt,Oliver Batistič,Jörg Kudla +9 more
TL;DR: A new set of 57 vectors that enable transgene expression in transiently or stably transformed cells and investigated the influence of CBL (Calcineurin B-like) protein expression on the subcellular localization of CIPKs, finding that induced co-expression of C BL3 is fully sufficient for dynamic translocation of C IPK5 from the cytoplasm to the tonoplast.
Journal ArticleDOI
Structural Biology of a Major Signaling Network that Regulates Plant Abiotic Stress: The CBL-CIPK Mediated Pathway.
TL;DR: The Arabidopsis SOS2 family of twenty-six protein kinases, their interacting activators, the SOS3 family of ten calcium-binding proteins and protein phosphatases type 2C, function together in decoding calcium signals elicited by different environmental stimuli.
Journal ArticleDOI
Integration of Abscisic Acid Signaling with Other Signaling Pathways in Plant Stress Responses and Development.
TL;DR: The perception of the stress signal (abiotic and biotic) and the response network of ABA signaling components that transduce the signal to the downstream pathway to respond to stress tolerance, regulation of stomata, and A BA signaling component ubiquitination are examined.
References
More filters
Journal ArticleDOI
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
TL;DR: A novel multienzyme approach was used to generate a set of highly representative genomic libraries from S. cerevisiae and a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter.
Journal ArticleDOI
ROS signaling: the new wave?
Ron Mittler,Ron Mittler,Sandy Vanderauwera,Nobuhiro Suzuki,Gad Miller,Vanesa B. Tognetti,Klaas Vandepoele,Marty Gollery,Vladimir Shulaev,Frank Van Breusegem +9 more
TL;DR: This review will attempt to address several key questions related to the use of ROS as signaling molecules in cells, including the dynamics and specificity of ROS signaling, networking of ROS with other signaling pathways, ROS signaling within and across different cells, ROS waves and the evolution of the ROS gene network.
Journal ArticleDOI
NADPH oxidase AtrbohD and AtrbohF genes function in ROS‐dependent ABA signaling in Arabidopsis
June M. Kwak,Izumi C. Mori,Zhen-Ming Pei,Nathalie Leonhardt,Miguel Angel Torres,Jeffery L. Dangl,Rachel E. Bloom,Sara Bodde,Jonathan D. G. Jones,Julian I. Schroeder +9 more
TL;DR: Identification of two partially redundant Arabidopsis guard cell‐expressed NADPH oxidase catalytic subunit genes, AtrbohD and atrbohF, are reported, providing direct molecular genetic and cell biological evidence that ROS are rate‐limiting second messengers in ABA signaling, and that the AtrbOHD and AtrabohF NADPH oxidationases function in guard cell ABA signal transduction.
Journal ArticleDOI
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
Michael Walter,Christina Chaban,Katia Schütze,Oliver Batistič,Katrin Weckermann,Christian Näke,Dragica Blazevic,Christopher Grefen,Karin Schumacher,Claudia Oecking,Klaus Harter,Jörg Kudla +11 more
TL;DR: The implementation of a bimolecular fluorescence complementation technique for visualization of protein-protein interactions in plant cells revealed a remarkable signal fluorescence intensity of interacting protein complexes as well as a high reproducibility and technical simplicity of the method in different plant systems.
Journal ArticleDOI
Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response
TL;DR: In this paper, the authors analyzed lines carrying dSpm insertions in the highly expressed AtrbohD and AtrabohF genes, which are required for full ROI production observed during incompatible interactions with the bacterial pathogen Pseudomonas syringae pv.