The chromatographic identification of some biologically important phosphate esters.
TLDR
The objective of the present work was to provide a means for separating and indentifying phosphate esters involved in glycolysis in higher plants using two-dimensional chromatography with successive development in an acid and in a basic solvent.About:
This article is published in Journal of Biological Chemistry.The article was published on 1951-11-01 and is currently open access. It has received 675 citations till now.read more
Citations
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Journal ArticleDOI
A column chromatographic separation of classes of phospholipides.
Journal ArticleDOI
The biosynthesis of trehalose phosphate
Enrico Cabib,Luis F. Leloir +1 more
TL;DR: Crystalline barium glucose g-phosphate was obtained from the Sigma Chemical Company, St. Louis, and intestinal phosphatase from Armour and Company, Chicago and the phosphat enzyme was dialyzed overnight against cold distilled water before use.
Journal ArticleDOI
Aspartate Transcarbamylase INTERACTION WITH THE TRANSITION STATE ANALOGUE N-(PHOSPHONACETYL)-l-ASPARTATE
Kim D. Collins,George R. Stark +1 more
TL;DR: The interaction of PALA with the enzyme is consistent with the "compression" model for aspartate transcarbamylase previously proposed and puts the enzyme into the same contracted conformation as do carbamyl phosphate and succinate (an analogue of l-aspartate) acting together.
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Human fibrinopeptides. Isolation, characterization and structure.
TL;DR: The structure of human fibrinogen and the specificity of thrombin is discussed, with a focus on the A-, AP- and Y-peptides.
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Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets.
TL;DR: The addition of thrombin to human platelets prelabeled with 32Pi led to significant loss of radioactivity in phosphatidylinositol 4,5-bisphosphate within 5 s, followed by recovery or even increase by 2 min, which suggests that a pool of polyphosphoinositides is constantly undergoing phosphodiesteratic cleavage and resynthesis.
References
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Journal ArticleDOI
Separation of the Phosphoric Esters on the Filter Paper Chromatogram
C. S. Hanes,F. A. Isherwood +1 more
TL;DR: Progress in this important field has depended upon the development of methods for identifying and determining the individual esters present in mixtures, but these methods frequently fail to yield diagnostic results.
Journal ArticleDOI
Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall.
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Aniline Hydrogen Phthalate as a Spraying Reagent for Chromatography of Sugars
TL;DR: The use of ammoniacal silver nitrate solution as a spraying reagent for revealing the presence of sugars on filter-paper chromatograms has the advantage of general application but has a corresponding disadvantage in reacting with a very wide range of reducing substances other than the sugars, including various impurities commonly present in such solvents as phenol and collidine.
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The path of carbon in photosynthesis. V. Paper chromatography and radioautography of the products.
TL;DR: Paper chromatography has been used in the identification of compounds formed by plants during short periods of photosynthesis as mentioned in this paper, and it has been shown to be useful in the separation of polycarboxylic acids and phosphate esters.
Related Papers (5)
Separation of the Phosphoric Esters on the Filter Paper Chromatogram
C. S. Hanes,F. A. Isherwood +1 more