The complete genome of the crenarchaeon Sulfolobus solfataricus P2.
Qunxin She,Rama K. Singh,Fabrice Confalonieri,Yvan Zivanovic,Ghislaine Allard,Mariana J. Awayez,Christina C.-Y. Chan-Weiher,Ib Groth Clausen,Bruce A. Curtis,Anick De Moors,Gaël Erauso,Cynthia Fletcher,Paul M. K. Gordon,Ineke Heikamp-de Jong,Alex C. Jeffries,Catherine Kozera,Nadine Medina,Xu Peng,Hoa Phan Thi-Ngoc,Peter Redder,Margaret E. Schenk,Cynthia Theriault,Niels Tolstrup,Robert L. Charlebois,W. Ford Doolittle,Michel Duguet,Terry Gaasterland,Roger A. Garrett,Mark A. Ragan,Christoph Wilhelm Sensen,John van der Oost +30 more
TLDR
The results illustrate major differences between crenarchaea and euryarchaea, especially for their DNA replication mechanism and cell cycle processes and their translational apparatus.Abstract:
The genome of the crenarchaeon Sulfolobus solfataricus P2 contains 2,992,245 bp on a single chromosome and encodes 2,977 proteins and many RNAs. One-third of the encoded proteins have no detectable homologs in other sequenced genomes. Moreover, 40% appear to be archaeal-specific, and only 12% and 2.3% are shared exclusively with bacteria and eukarya, respectively. The genome shows a high level of plasticity with 200 diverse insertion sequence elements, many putative nonautonomous mobile elements, and evidence of integrase-mediated insertion events. There are also long clusters of regularly spaced tandem repeats. Different transfer systems are used for the uptake of inorganic and organic solutes, and a wealth of intracellular and extracellular proteases, sugar, and sulfur metabolizing enzymes are encoded, as well as enzymes of the central metabolic pathways and motility proteins. The major metabolic electron carrier is not NADH as in bacteria and eukarya but probably ferredoxin. The essential components required for DNA replication, DNA repair and recombination, the cell cycle, transcriptional initiation and translation, but not DNA folding, show a strong eukaryal character with many archaeal-specific features. The results illustrate major differences between crenarchaea and euryarchaea, especially for their DNA replication mechanism and cell cycle processes and their translational apparatus.read more
Citations
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Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
TL;DR: It is shown that CRISPR spacers derive from preexisting sequences, either chromosomal or within transmissible genetic elements such as bacteriophages and conjugative plasmids, implying a relationship betweenCRISPR and immunity against targeted DNA.
Journal ArticleDOI
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.
TL;DR: CRISPRFinder is described, a web service offering tools to detect CRISPRs including the shortest ones including one or two motifs, define DRs and extract spacers, and get the flanking sequences to determine the leader.
Journal ArticleDOI
The Sir2 Family of Protein Deacetylases
Gil Blander,Leonard Guarente +1 more
TL;DR: The role of NAD+, the unusual products of the deacetylation reaction, the Sir2 structure, and the Sir1 and Sir2 chemical inhibitors and activators that were recently identified are discussed.
Journal ArticleDOI
A Guild of 45 CRISPR-Associated (Cas) Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes
TL;DR: It is evident from this analysis that CRISPR/cas loci are larger, more complex, and more heterogeneous than previously appreciated.
Journal ArticleDOI
Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil
Maria Tourna,Michaela Stieglmeier,Anja Spang,Martin Könneke,Arno Schintlmeister,Tim Urich,Marion Engel,Michael Schloter,Michael Wagner,Andreas Richter,Christa Schleper +10 more
TL;DR: The cultivation and isolation of an AOA from soil is described, showing it grows on ammonia or urea as an energy source and is capable of using higher ammonia concentrations than the marine isolate, Nitrosopumilus maritimus.
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