Journal ArticleDOI
The coupling of electron transfer in nitrogenase to the hydrolysis of magnesium adenosine triphosphate.
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This article is published in Biochemical Society Transactions.The article was published on 1979-08-01. It has received 21 citations till now. The article focuses on the topics: ATP hydrolysis & Electron transfer.read more
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Mössbauer, EPR, and magnetization studies of the Azotobacter vinelandii Fe protein. Evidence for a [4Fe-4S]1+ cluster with spin S = 3/2.
TL;DR: The studies show that reduced Av2 in the native state is heterogeneous, which explains why the integrated spin intensity of the g = 1.94 EPR signal is substantially less than 1 spin/4 Fe atoms.
Journal ArticleDOI
Electron Transfer in Nitrogenase
Hannah L Rutledge,F. Akif Tezcan +1 more
TL;DR: This review focuses on the structural and thermodynamic redox properties of nitrogenase component proteins and their complexes, as well as on new insights regarding the mechanism of ET reactions during catalysis and how they are coupled to ATP hydrolysis.
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Electron Transfer within Nitrogenase: Evidence for a Deficit-Spending Mechanism
TL;DR: The data for the β-153(Cys) deletion MoFe protein variant provide an argument against an alternative two-step "hopping" ET model that reverses the two ET steps, with the Fe protein first transferring an electrons to the P cluster, which in turn transfers an electron to FeMo-cofactor.
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Electron transfer precedes ATP hydrolysis during nitrogenase catalysis
Simon Duval,Karamatullah Danyal,Sudipta Shaw,Anna K. Lytle,Dennis R. Dean,Brian M. Hoffman,Edwin Antony,Edwin Antony,Lance C. Seefeldt,Lance C. Seefeldt +9 more
TL;DR: This work establishes the order of all the key events during one catalytic cycle of electron delivery in nitrogenase, showing that ATP hydrolysis follows electron transfer, and guides future studies aimed at understanding what roles ATP binding and hydroleysis play in the nitrogenase mechanism.
Journal ArticleDOI
Evidence That the Pi Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle
Zhi-Yong Yang,Rhesa N. Ledbetter,Sudipta Shaw,Natasha Pence,Monika Tokmina-Lukaszewska,Brian J. Eilers,Qingjuan Guo,Nilisha Pokhrel,Valerie L. Cash,Dennis R. Dean,Edwin Antony,Brian Bothner,John W. Peters,Lance C. Seefeldt +13 more
TL;DR: The findings indicate that with this reductant, the rate-limiting step in the reaction cycle is not protein-protein dissociation or reduction of the oxidized Fe protein, but rather events associated with the Pi release step.