Journal ArticleDOI
The crystal structure of the bacterial chaperonin GroEL at 2.8 A.
Kerstin Braig,Zbyszek Otwinowski,Zbyszek Otwinowski,Rashmi Hegde,Rashmi Hegde,David C Boisvert,Andrzej Joachimiak,Andrzej Joachimiak,Arthur L. Horwich,Paul B. Sigler +9 more
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TLDR
The crystal structure of Escherichia coli GroEL shows a porous cylinder of 14 subunits made of two nearly 7-fold rotationally symmetrical rings stacked back-to-back with dyad symmetry.Abstract:
The crystal structure of Escherichia coli GroEL shows a porous cylinder of 14 subunits made of two nearly 7-fold rotationally symmetrical rings stacked back-to-back with dyad symmetry. The subunits consist of three domains: a large equatorial domain that forms the foundation of the assembly at its waist and holds the rings together; a large loosely structured apical domain that forms the ends of the cylinder; and a small slender intermediate domain that connects the two, creating side windows. The three-dimensional structure places most of the mutationally defined functional sites on the channel walls and its outward invaginations, and at the ends of the cylinder.read more
Citations
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Journal ArticleDOI
Molecular chaperones in cellular protein folding.
TL;DR: Significant progress has been made in the understanding of the ATP-dependent mechanisms used by the Hsp70 and chaperonin families of molecular chaperones, which can cooperate to assist in folding new polypeptide chains.
Journal ArticleDOI
The Hsp70 and Hsp60 chaperone machines.
Bernd Bukau,Arthur L. Horwich +1 more
TL;DR: This work dedicates this work to Guenter Brueckner, always an inspiration, and to Wayne Fenton for critical reading and Zhaohui Xu for figure preparation.
Journal ArticleDOI
Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution.
TL;DR: The three-dimensional structure of the proteasome from the archaebacterium Thermoplasma acidophilum has been elucidated by x-ray crystallographic analysis by means of isomorphous replacement and cyclic averaging.
Journal ArticleDOI
Automated molecular microscopy: the new Leginon system.
Christian Suloway,James Pulokas,Denis Fellmann,Anchi Cheng,Francisco Guerra,Joel Quispe,Scott M. Stagg,Clinton S. Potter,Bridget Carragher +8 more
TL;DR: The primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years and the system has demonstrated the capacity for high throughput data acquisition by acquiring images of more than 100,000 particles in a single session at the microscope.
Journal ArticleDOI
Absolute Quantification of Proteins by LCMSE A Virtue of Parallel ms Acquisition
TL;DR: A new method of absolute quantification of proteins is described, based on the discovery of an unexpected relationship between MS signal response and protein concentration, which is constant within a coefficient of variation of less than ±10%.
References
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Improved Fourier coefficients for maps using phases from partial structures with errors
TL;DR: In this article, a method is given to estimate the parameter σA in these phase probability expressions from the observed and calculated structure factor amplitudes, from which one can estimate the mean coordinate error for the model, and when there are coordinate errors, a new expression for the non-centric Fourier coefficients is required to suppress this model bias.