The DEFECTIVE IN ANTHER DEHISCENCE1 Gene Encodes a Novel Phospholipase A1 Catalyzing the Initial Step of Jasmonic Acid Biosynthesis, Which Synchronizes Pollen Maturation, Anther Dehiscence, and Flower Opening in Arabidopsis
TLDR
Results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis and is restricted in the stamen filaments.Abstract:
The Arabidopsis mutant defective in anther dehiscence1 (dad1) shows defects in anther dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1-specific manner, and DAD1-green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.read more
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Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany
Claus Wasternack,Bettina Hause +1 more
TL;DR: Important new components of jasmonate signalling including its receptor were identified, providing deeper insight into the role ofJASMONATE signalling pathways in stress responses and development.
Journal ArticleDOI
Jasmonates: An Update on Biosynthesis, Signal Transduction and Action in Plant Stress Response, Growth and Development
TL;DR: This review summarizes biosynthesis and signal transduction of jasmonates with emphasis on new findings in relation to enzymes, their crystal structure, new compounds detected in the oxylipin andJasmonate families, and newly found functions.
Journal ArticleDOI
The lipoxygenase pathway.
Ivo Feussner,Claus Wasternack +1 more
TL;DR: Activation of this pathway as an initial step in the interaction of plants with pathogens, insects, or abiotic stress and at distinct stages of development are reviewed.
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Acyl-Lipid Metabolism
Younghua Li-Beisson,Basil S. Shorrosh,Fred Beisson,Mats X. Andersson,Vincent Arondel,Philip D. Bates,Sébastien Baud,David Bird,Debono Allan George,Timothy P. Durrett,Rochus Franke,Ian A. Graham,Kenta Katayama,Amélie A. Kelly,Tony R. Larson,Jonathan E. Markham,Martine Miquel,Isabel Molina,Ikuo Nishida,Owen Rowland,Lacey Samuels,Katherine M. Schmid,Hajime Wada,Ruth Welti,Changcheng Xu,Rémi Zallot,John B. Ohlrogge +26 more
TL;DR: This chapter focuses on the metabolic pathways that are associated with the biosynthesis and degradation of the acyl lipids that represent their major form of carbon and energy storage in Arabidopsis.
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The Jasmonate Signal Pathway
TL;DR: Recent advances in understanding of the role of JAs in plant responses to biotic and abiotic stresses are reviewed.
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