The human ubiquitin gene family: structure of a gene and pseudogenes from the Ub B subfamily
Rohan T. Baker,Philip G. Board +1 more
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TLDR
Hybridisation analysis of restricted human genomic DNA suggests the presence of one other closely related gene within the genome.Abstract:
An ubiquitin cDNA clone was isolated from a human liver cDNA library. This clone contained two complete, and a portion of a third, ubiquitin coding sequences joined head to tail with no spacer peptides. Screening a human genomic library with a probe derived from the coding region of this cDNA identified a large number of cross-hybridising clones. Differential screening of these genomic clones with the 3' non-coding region of the cDNA identified three different 3'-positive clones. Sequence analysis of these three clones revealed: a gene corresponding to the cDNA containing an intron in the 5' non-coding region and coding for three direct repeats of mature ubiquitin, and two related pseudogenes which appear to have arisen by reverse transcription and insertion into the genome. However, one pseudogene contains two repeats of the ubiquitin coding sequence, while the other contains only one. Hybridisation analysis of restricted human genomic DNA suggests the presence of one other closely related gene within the genome.read more
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Regulation and Cellular Roles of Ubiquitin-Specific Deubiquitinating Enzymes
TL;DR: A review of recent studies of the regulation of DUB activity and their roles in various cellular processes can be found in this paper, where the authors discuss ubiquitin-specific DUBs and some of the generalizations emerging from recent studies.
Journal ArticleDOI
Frameshift Mutants of β Amyloid Precursor Protein and Ubiquitin-B in Alzheimer's and Down Patients
Fred W. van Leeuwen,Dominique P.V. de Kleijn,Helma H. van den Hurk,Andrea Neubauer,M. A. F. Sonnemans,Jacqueline A. Sluijs,Soner Köycü,Ravindra D. J. Ramdjielal,Ahmad Salehi,Gerard J.M. Martens,Frank Grosveld,J. Peter H. Burbach,Elly M. Hol +12 more
TL;DR: The cerebral cortex of Alzheimer's and Down syndrome patients is characterized by the presence of protein deposits in neurofibrillary tangles, neuritic plaques, and neuropil threads.
Journal ArticleDOI
The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression
TL;DR: An intron in the 5′-untranslated region at the same position immediately upstream of the initiator methionine codon in each of the three Arabidopsis thaliana polyubiquitin genes is revealed.
Journal ArticleDOI
Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family.
TL;DR: YUH1 and UBP1, the genes for two ubiquitin-specific proteases of the yeast Saccharomyces cerevisiae, have been cloned previously and shown to encode nonhomologous proteins, but using an Escherichia coli-based genetic screen, two other yeast genes are isolated, named UBP2 and U BP3, indicating the presence of at least one more ubiquitIn-specific processing protease in S. cerevisia.
Journal ArticleDOI
TNF and ubiquitin at the crossroads of gene activation, cell death, inflammation, and cancer.
TL;DR: In this paper, the authors focus on the molecular and physiological consequences of the interaction of TNF with TNF receptor 1 (TNFR1), and develop a new concept for the way the concerted action of different ubiquitination events enables the TNF-RSC to generate its signaling output in a spatio-temporally controlled manner.
References
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Book
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.