Journal ArticleDOI
The JAM test A simple assay for DNA fragmentation and cell death
TLDR
A method is described, based on the recent evidence that dying cells often degrade their DNA into small fragments, which measures the DNA retained by living cells rather than the cellular components lost by dying cells.About:
This article is published in Journal of Immunological Methods.The article was published on 1991-12-15. It has received 641 citations till now. The article focuses on the topics: DNA fragmentation & Lysis.read more
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CD4 + T cells are required for secondary expansion and memory in CD8 + T lymphocytes
Edith M. Janssen,Edward E. Lemmens,Tom Wolfe,Urs Christen,Matthias von Herrath,Stephen P. Schoenberger +5 more
TL;DR: The results demonstrate that T-cell help is ‘programmed’ into CD8+ T cells during priming, conferring on these cells a hallmark of immune response memory: the capacity for functional expansion on re-encounter with antigen.
Journal ArticleDOI
Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas
Thomas Brunner,Rona J. Mogil,Drake LaFace,Nam Jin Yoo,Artin Mahboubi,Fernando Echeverri,Seamus J. Martin,Walker R. Force,David H. Lynch,Carl F. Ware,Douglas R. Green +10 more
TL;DR: This work shows that the Fas/CD95 receptor, which can transduce a potent apoptotic signal when ligated, is rapidly expressed following activation of T-cell hybridomas, as is its functional, membrane-bound ligand8.
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The fas counterattack : fas-mediated T cell killing by colon cancer cells expressing fas ligand
TL;DR: A Fas counterattack model for immune escape in colon cancer is suggested, whereby the cancer cells resist Fas-mediated T cell cytotoxicity but express functional FasL, an apoptotic death signal to which activated T cells are inherently sensitive.
Journal ArticleDOI
Naïve CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation
TL;DR: It is found that naïve CTLs become committed after as little as 2 h of exposure to APCs and that their subsequent division and differentiation can occur without the need for further antigenic stimulation of the daughter cells, whether priming is in vitro or in vivo.
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Neonatal Tolerance Revisited: Turning on Newborn T Cells with Dendritic Cells
TL;DR: Reexamination of the classic Neonatal tolerance experiments showed that tolerance is not an intrinsic property of the newborn immune system, but that the nature of the antigen-presenting cell determines whether the outcome is neonatal tolerance or immunization.
References
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Journal Article
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
TL;DR: The in vitro cytotoxic effect of spleen cells of mice immunized by tumour allografts was studied by measuring target cell inactivation as a function of release of radioactive label (51Cr) or loss of cloning efficiency.
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Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
TL;DR: It is found that Zn2+ also inhibits DNA fragmentation and 51Cr release induced by cytotoxic T cells, suggesting a final common biochemical pathway for both types of cell death.
Journal Article
Differences in target cell DNA fragmentation induced by mouse cytotoxic T lymphocytes and natural killer cells.
TL;DR: The results suggest that NK cells induce target cell injury via two different mechanisms, one of which would involve lysis mediated by cell-to-cell contact, while the other may induce DNA fragmentation via a soluble mediator.
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Antigen-dependent stimulation of synthesis of deoxyribonucleic acid in spleen cells from immunized rabbits.
TL;DR: The experiments described here demonstrate that the addition of antigen to the incubation medium stimulates the synthesis of DNA in spleen cells from previously immunized rabbits.