The lipopolysaccharide of a chloridazon-degrading bacterium.
Rüdiger Weisshaar,Franz Lingens +1 more
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TLDR
Dodecanoic acid was demonstrated to be linked with the hydroxy groups of the amide-bound fatty acids of a chloridazon-degrading bacterium, and the taxonomic significance of 2,3-diamino-2, 3-dideoxy-D-glucose, is discussed.Abstract:
Lipopolysaccharide of a chloridazon-degrading bacterium was obtained by a two-stage extraction procedure with phenol/EDTA in a yield of 0.3% of dried bacteria. The carbohydrate moiety consisted of heptose, 3-deoxyoctulosonic acid and D-glucose in a molar ratio of 1:2:2 X 3. Lipid A was composed of 1 mol 2,3-diamino-2,3-dideoxy-D-glucose, 2 mol amide-bound and 2.6 mol ester-bound fatty acids/mol. Amide-bound fatty acids were 3-hydroxydodecanoic acid and 3-hydroxyhexadecanoic acid; dodecanoic acid and R-(-)-3-hydroxydodec-5-cis-enoic acid were found to be present in ester linkage. Under conditions of acidic hydrolysis, the latter was converted into the cis and trans isomers of 5-hexyltetrahydrofuran-2-acetic acid. Dodecanoic acid was demonstrated to be linked with the hydroxy groups of the amide-bound fatty acids. The taxonomic significance of these results, especially the demonstration of 2,3-diamino-2, 3-dideoxy-D-glucose, is discussed.read more
Citations
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Book ChapterDOI
Molecular structure of lipid A, the endotoxic center of bacterial lipopolysaccharides.
Journal ArticleDOI
Phenylobacterium immobile gen. nov., sp. nov., a Gram-Negative Bacterium That Degrades the Herbicide Chloridazon
Franz Lingens,Renate Blecher,Hartmut Blecher,Friedhelm Blobel,Jürgen Eberspächer,Christoph Fröhner,Helma Görisch,Helmut Görisch,Gerlinde Layh +8 more
TL;DR: Bacteria which utilize the xenobiotic compounds chloridazon, antipyrin, and pyramidon as sole carbon sources were isolated from various soil samples and characterized as a new genus, Phenylobacterium, with a single species,phenylobacteria immobile, which is a serologically uniform group of organisms, which are harmless to rats and rabbits.
Book ChapterDOI
6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria
TL;DR: The terms lipopolysaccharide, O-antigen, and endotoxin are used synonymously and describe either the chemical structure or discrete biological functions of the same substance.
Journal ArticleDOI
Structural analysis of the lipid A component of Campylobacter jejuni CCUG 10936 (serotype O:2) lipopolysaccharide. Description of a lipid A containing a hybrid backbone of 2-amino-2-deoxy-D-glucose and 2,3-diamino-2,3-dideoxy-D-glucose.
Anthony P. Moran,Ulrich Zähringer,Ulrich Seydel,Dieter Scholz,Peter Stütz,Ernst Th. Rietschel +5 more
TL;DR: The chemical structure of Campylobacter jejuni CCUG 10936 lipid A was elucidated and by methylation analysis it was shown that position 6' is the attachment site for the polysaccharide moiety in lipopolysaccharid.
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