Journal ArticleDOI
The mechanism of the bleomycin-induced cleavage of DNA.
J. William Lown,Soo-Khoon Sim +1 more
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It is shown that 2-Mercaptoethanol, dithiothreitol, ascorbic acid and xanthine/xanthine oxidase, hitherto commonly used with bleomycin studies, separately cleave DNA by a similar mechanism.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1977-08-22. It has received 242 citations till now. The article focuses on the topics: Superoxide & Xanthine oxidase.read more
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Superoxide Radical and Superoxide Dismutases
TL;DR: O2- oxidizes the [4Fe-4S] clusters of dehydratases, such as aconitase, causing-inactivation and release of Fe(II), which may then reduce H2O2 to OH- +OH..
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Intercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II.
TL;DR: The agreement between the in vivo and in vitro studies suggests that mammalian DNA topoisomerase II is a drug target in vivo, and the cytotoxic action of epipodophyllotoxins may be analogous to the bactericidal action of nalidixic acid.
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Murine models of pulmonary fibrosis
TL;DR: The characteristics, advantages, and disadvantages of many of the currently utilized murine models of pulmonary fibrosis are summarized.
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Short-term tests for carcinogens and mutagens
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Classification of Antineoplastic Agents by their Selective Toxicities toward Oxygenated and Hypoxic Tumor Cells
TL;DR: Future chemotherapeutic regimens for the treatment of solid tumors should include agents and modalities directed toward the hypoxic cell population of the tumor, as well as toward the proliferating and nonproliferating tumor cell compartments.
References
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Journal Article
New antibiotics, bleomycin A and B.
Journal ArticleDOI
Generation of superoxide free radical during the autoxidation of thiols.
TL;DR: It is proposed that thiols reduce nitroblue tetrazolium by at least two different pathways, one of which involves O2- and is therefore inhibitable by superoxide dismutase.
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A role for ferrous ion and oxygen in the degradation of DNA by bleomycin.
TL;DR: An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported and optical spectral studies reveal that an oxygen-labile complex is formed between bleomerscin and Fe( II).
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Increased DNA chain breakage by combined action of bleomycin and superoxide radical
R. Ishida,T. Takahashi +1 more
TL;DR: In vitro DNA chain breakage by bleomycin was enhanced by the addition of xanthine oxidase system and it is concluded that superoxide radical is one of the chemical mediators responsible for the enhancement of the DNAChain breakage action ofBleomycin.
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The mechanism of the degradation of DNA by streptonigrin.
TL;DR: The production of single strand cleavage in covalently-closed circular-DNA by the antitumour agent streptonigrin (reduced in situ by NADH) is demonstrated using the ethidium bromide fluorescence assay described previously.