The selection of S. cerevisiae mutants defective in the start event of cell division.
TLDR
Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cerevisiae were isolated and subjected to preliminary characterization, revealing single nuclear genes, unlinked to one another.Abstract:
Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cerevisiae were isolated and subjected to preliminary characterization. Complementation studies assigned thes mutations to four complementation groups, one of which, cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes, cdc37, has been located in the yeast linkage map on chromosome IV, two meiotic map units distal to hom2.--Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.read more
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Tyrosine phosphorylation of the fission yeast cdc2 + protein kinase regulates entry into mitosis
Kathleen L. Gould,Paul Nurse +1 more
TL;DR: The cdc2+ protein kinase (pp34) is found to be phosphorylated on tyrosine as well as serine and threonine residues in exponentially growing Schizosaccharomyces pombe, establishing that tyrosines phosphorylation/dephosphorylation directly regulates pp34 function.
Journal ArticleDOI
Regulation of retinoblastoma protein functions by ectopic expression of human cyclins
Philip W. Hinds,Sibylle Mittnacht,Vjekoslav Dulic,Andrew Arnold,Steven I. Reed,Robert A. Weinberg +5 more
TL;DR: It is shown that constitutively expressed cyclins A and E can overcome pRB-mediated suppression of proliferation and suggest that G1 and S phase cyclins can act as regulators of pRb function in the cell cycle by promoting pR b phosphorylation.
Journal ArticleDOI
A chemical switch for inhibitor-sensitive alleles of any protein kinase
Anthony C. Bishop,Jeffrey A. Ubersax,Dejah T. Petsch,Dina Matheos,Nathanael S. Gray,Justin D. Blethrow,Eiji Shimizu,Joe Z. Tsien,Peter G. Schultz,Mark D. Rose,John L. Wood,David O. Morgan,Kevan M. Shokat,Kevan M. Shokat +13 more
TL;DR: A chemical genetic strategy for sensitizing protein kinases to cell-permeable molecules that do not inhibit wild-type kinases is described, allowing for rapid functional characterization of members of this important gene family.
Journal ArticleDOI
Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression
Jeannette Kunz,Ruben Henriquez,Ulrich Schneider,Maja Deuter-Reinhard,N.Rao Movva,Michael N. Hall +5 more
TL;DR: The results suggest that 3-phosphorylated phosphoinositides, whose physiological significance has not been determined, are an important signal in cell cycle activation in yeast and may act in a signalTransduction pathway similar to the interleukin-2 signal transduction pathway in T cells.
Journal ArticleDOI
Physical interaction of the retinoblastoma protein with human D cyclins
Steven F. Dowdy,Philip W. Hinds,Kenway Louie,Steven I. Reed,Andrew Arnold,Robert A. Weinberg +5 more
TL;DR: It is concluded that cyclins D1 and D3 interact with pRb in a fashion distinct from cyclins A and E, which can induce pRB hyperphosphorylation, and that cyclin D1 activity may be regulated by its association with p Rb.
References
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Journal ArticleDOI
Coordination of growth with cell division in the yeast Saccharomyces cerevisiae
TL;DR: It is proposed that the normal coordination of cell growth with cell division is a consequence of the following two relationships: growth, rather than progress through the DNA-division cycle, is normally rate-limiting for cell proliferation and a specific early event in G1 cannot be completed until a critical size is attained.
Journal ArticleDOI
GENETIC CONTROL OF THE CELL DIVISION CYCLE IN YEAST: V. GENETIC ANALYSIS OF cdc MUTANTS
TL;DR: The gene products that are defined by the cdc cistrons are essential for the completion of the cell cycle in haploids of a and alpha mating type and in a/alpha diploid cells and the same genes control thecell cycle in each of these stages of the life cycle.
Journal ArticleDOI
Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast
TL;DR: The mutants were tested for loss of viability, change in morphology, increase in cell number, and ability to synthesize protein, ribonucleic acid (RNA), and deoxyribonuclear acid (DNA) after a shift from 23 to 36 C as mentioned in this paper.
Journal ArticleDOI
Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division.
TL;DR: The budding yeast, Saccharomyces cerevisiae, was grown exponentially at different rates in the presence of growth rate-limiting concentrations of a protein synthesis inhibitor, cycloheximide, to support the model for the coordination of growth and division.
Book ChapterDOI
The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.
D H Williamson,D J Fennell +1 more
TL;DR: The use of 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) in cesium chloride gradients, fluorescent staining of cells with DAPI, and sensitivity of the staining procedure are discussed.