Journal ArticleDOI
The use of molecular techniques to characterize the microbial communities in contaminated soil and water
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TLDR
This review examines the current application of molecular techniques for the characterization of microbial communities in contaminated soil and water and methods that directly link microbial phylogeny to its ecological function at contaminated sites as well as high throughput methods for complex microbial community studies.About:
This article is published in Environment International.The article was published on 2008-02-01. It has received 204 citations till now. The article focuses on the topics: Microbial population biology & Bioremediation.read more
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Bioremediation approaches for organic pollutants: a critical perspective
Mallavarapu Megharaj,Balasubramanian Ramakrishnan,Balasubramanian Ramakrishnan,Balasubramanian Ramakrishnan,Kadiyala Venkateswarlu,Kadiyala Venkateswarlu,Kadiyala Venkateswarlu,N. Sethunathan,Ravi Naidu,Ravi Naidu +9 more
TL;DR: This review selectively examines and provides a critical view on the knowledge gaps and limitations in field application strategies, approaches such as composting, electrobioremediation and microbe-assisted phytoremediating, and the use of probes and assays for monitoring and testing the efficacy of bioremediations of polluted sites.
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Diagnosis of soil contamination using microbiological indices: A review on heavy metal pollution.
Jiayi Tang,Jiachao Zhang,Liheng Ren,Yaoyu Zhou,Jun Gao,Lin Luo,Yuan Yang,Qinghui Peng,Hongli Huang,Anwei Chen +9 more
TL;DR: The changes of different microbiological indices and the mechanism of microbial response to heavy metal stress in soils are comprehensively summarized and future directions of the microbial ecotoxicological diagnosis of soil contamination by heavy metals are proposed and discussed.
Journal ArticleDOI
Monitoring of microbial hydrocarbon remediation in the soil.
TL;DR: Microbial methods for monitoring bioremediation of hydrocarbons include chemical, biochemical and microbiological molecular indices that measure rates of microbial activities to show that in the end the target goal of pollutant reduction to a safe and permissible level has been achieved.
Journal ArticleDOI
Scientific Opinion addressing the state of the science on risk assessment of plant protection products for in-soil organisms
Colin Ockleford,Paulien Adriaanse,Philippe Berny,Theodorus Brock,Sabine Duquesne,Sandro Grilli,Antonio F Hernandez-Jerez,Susanne Hougaard Bennekou,Michael Klein,Thomas Kuhl,Ryszard Laskowski,Kyriaki Machera,Olavi Pelkonen,Silvia Pieper,Michael Stemmer,Ingvar Sundh,Ivana Teodorovic,Aaldrik Tiktak,Christopher J. Topping,Gerrit Wolterink,Peter S. Craig,Frank de Jong,Barbara Manachini,Paulo Sousa,Klaus Swarowsky,Domenica Auteri,Maria Arena,Smith Rob +27 more
TL;DR: It is proposed that in‐soil environmental risk assessments are made at in‐ and off‐field scale considering field boundary levels, and a new testing strategy which takes into account the relevant exposure routes for in‐ soil organisms and the potential direct and indirect effects is proposed.
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Nanoliter multiplex PCR arrays on a SlipChip
Feng Shen,Wenbin Du,Elena K. Davydova,Mikhail A. Karymov,Janmajay Pandey,Rustem F. Ismagilov +5 more
TL;DR: The SlipChip will be useful for applications involving PCR arrays and lays the foundation for new strategies for diagnostics, point-of-care devices, and immobilization-based arrays.
References
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Molecular techniques in wastewater: Understanding microbial communities, detecting pathogens, and real-time process control.
TL;DR: The current and emerging molecular approaches for characterizing microbial community composition and structure in wastewater processes are reviewed and the ability to automate a detection/identification system is discussed.
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Molecular Characterization of Bacterial Populations in Petroleum-Contaminated Groundwater Discharged from Underground Crude Oil Storage Cavities
TL;DR: The results indicate that the novel members of the epsilon subclass of the Proteobacteria grow as major populations in the petroleum-contaminated cavity groundwater.
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Quantitative Detection of Microbial Genes by Using DNA Microarrays
Jae Chang Cho,James M. Tiedje +1 more
TL;DR: This approach for designing quantitative microarrays and the inferred equation from this study provide a simple and convenient way to estimate the target gene concentration from the hybridization signal ratio.
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RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater.
TL;DR: Results indicate that the Azoarcus population was involved in benzene degradation in the gasoline-contaminated groundwater under denitrifying conditions and suggest that RNA-based SIP identification coupled to phylogenetic screening of nonselective isolates facilitates the isolation of enrichment/isolation-resistant microorganisms with a specific function.
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Linking microbial community structure with function: fluorescence in situ hybridization-microautoradiography and isotope arrays.
TL;DR: The scope of FISH-MAR was extended by rendering it quantitative and by combining it with microelectrode measurements or stable isotope probing, and isotope arrays have also been developed that exploit the parallel detection offered by DNA microarrays to measure incorporation of labelled substrate into the rRNA of many community members in a single experiment.