Journal ArticleDOI
The use of molecular techniques to characterize the microbial communities in contaminated soil and water
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TLDR
This review examines the current application of molecular techniques for the characterization of microbial communities in contaminated soil and water and methods that directly link microbial phylogeny to its ecological function at contaminated sites as well as high throughput methods for complex microbial community studies.About:
This article is published in Environment International.The article was published on 2008-02-01. It has received 204 citations till now. The article focuses on the topics: Microbial population biology & Bioremediation.read more
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Bioremediation approaches for organic pollutants: a critical perspective
Mallavarapu Megharaj,Balasubramanian Ramakrishnan,Balasubramanian Ramakrishnan,Balasubramanian Ramakrishnan,Kadiyala Venkateswarlu,Kadiyala Venkateswarlu,Kadiyala Venkateswarlu,N. Sethunathan,Ravi Naidu,Ravi Naidu +9 more
TL;DR: This review selectively examines and provides a critical view on the knowledge gaps and limitations in field application strategies, approaches such as composting, electrobioremediation and microbe-assisted phytoremediating, and the use of probes and assays for monitoring and testing the efficacy of bioremediations of polluted sites.
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Diagnosis of soil contamination using microbiological indices: A review on heavy metal pollution.
Jiayi Tang,Jiachao Zhang,Liheng Ren,Yaoyu Zhou,Jun Gao,Lin Luo,Yuan Yang,Qinghui Peng,Hongli Huang,Anwei Chen +9 more
TL;DR: The changes of different microbiological indices and the mechanism of microbial response to heavy metal stress in soils are comprehensively summarized and future directions of the microbial ecotoxicological diagnosis of soil contamination by heavy metals are proposed and discussed.
Journal ArticleDOI
Monitoring of microbial hydrocarbon remediation in the soil.
TL;DR: Microbial methods for monitoring bioremediation of hydrocarbons include chemical, biochemical and microbiological molecular indices that measure rates of microbial activities to show that in the end the target goal of pollutant reduction to a safe and permissible level has been achieved.
Journal ArticleDOI
Scientific Opinion addressing the state of the science on risk assessment of plant protection products for in-soil organisms
Colin Ockleford,Paulien Adriaanse,Philippe Berny,Theodorus Brock,Sabine Duquesne,Sandro Grilli,Antonio F Hernandez-Jerez,Susanne Hougaard Bennekou,Michael Klein,Thomas Kuhl,Ryszard Laskowski,Kyriaki Machera,Olavi Pelkonen,Silvia Pieper,Michael Stemmer,Ingvar Sundh,Ivana Teodorovic,Aaldrik Tiktak,Christopher J. Topping,Gerrit Wolterink,Peter S. Craig,Frank de Jong,Barbara Manachini,Paulo Sousa,Klaus Swarowsky,Domenica Auteri,Maria Arena,Smith Rob +27 more
TL;DR: It is proposed that in‐soil environmental risk assessments are made at in‐ and off‐field scale considering field boundary levels, and a new testing strategy which takes into account the relevant exposure routes for in‐ soil organisms and the potential direct and indirect effects is proposed.
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Nanoliter multiplex PCR arrays on a SlipChip
Feng Shen,Wenbin Du,Elena K. Davydova,Mikhail A. Karymov,Janmajay Pandey,Rustem F. Ismagilov +5 more
TL;DR: The SlipChip will be useful for applications involving PCR arrays and lays the foundation for new strategies for diagnostics, point-of-care devices, and immobilization-based arrays.
References
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Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
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A Molecular View of Microbial Diversity and the Biosphere
TL;DR: Over three decades of molecular-phylogenetic studies, researchers have compiled an increasingly robust map of evolutionary diversification showing that the main diversity of life is microbial, distributed among three primary relatedness groups or domains: Archaea, Bacteria, and Eucarya.
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Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.
TL;DR: Computer-simulated analysis of terminal restriction fragment length polymorphisms (T-RFLP) for 1,002 eubacterial sequences showed that with proper selection of PCR primers and restriction enzymes, 686 sequences could be PCR amplified and classified into 233 unique terminal restriction fragments lengths or "ribotypes."
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Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
TL;DR: Results obtained with this approach indicate that a kinetic approach to PCR analysis can quantitate DNA sensitively, selectively and over a large dynamic range.
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Metagenomics: Application of Genomics to Uncultured Microorganisms
TL;DR: Reassembly of multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and microheterogeneity, and lateral gene transfer among members of an uncultured community.