Journal ArticleDOI
The use of molecular techniques to characterize the microbial communities in contaminated soil and water
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TLDR
This review examines the current application of molecular techniques for the characterization of microbial communities in contaminated soil and water and methods that directly link microbial phylogeny to its ecological function at contaminated sites as well as high throughput methods for complex microbial community studies.About:
This article is published in Environment International.The article was published on 2008-02-01. It has received 204 citations till now. The article focuses on the topics: Microbial population biology & Bioremediation.read more
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Dissertation
The Effect of Green Waste Composting on the Concentration and Composition of Ambient Bioaerosols
TL;DR: In this paper, the concentrations of bioaerosols upwind, on-site and downwind from two open-air green waste windrow composting facilities were enumerated in extensive detail, producing the first detailed and validated database of bio-aerosol concentrations at green-waste composting plants.
Journal ArticleDOI
Real-Time PCR Quantification of Protease-Producing Bacteria in Traditional Chinese Fish Sauce
TL;DR: The results may serve as a foundation for future studies on the microbial succession and variation of microorganisms during fish sauce fermentation and to calculate the number of protease-producing bacteria in fish sauce.
Journal ArticleDOI
Strain differentiation of airborne opportunistic microorganisms within a municipal landfill area as assessed by PCR MP method
K. Frączek,Jacek Kozdrój +1 more
TL;DR: This is the first report on the distribution of airborne opportunistic microbial species within the landfill area, performing a comparison of their genotypes and evaluation of genetic relatedness between the isolates using the PCR MP method.
Journal ArticleDOI
Bioprospecting marine actinomycetes for antileishmanial drugs: current perspectives and future prospects
O.F. Davies-Bolorunduro,O.F. Davies-Bolorunduro,O. Osuolale,S. Saibu,Isaac Adeyemi Adeleye,Nanik Siti Aminah +5 more
TL;DR: In this article, a review highlights metagenomic and bioassay approaches which could help streamline the drug discovery process thereby greatly reducing time and cost of dereplication to identify suitable antileishmanial drug candidates.
Journal ArticleDOI
An improved effective microorganism (EM) soil ball-making method for water quality restoration.
Gun-Seok Park,Abdur Rahim Khan,Yunyoung Kwak,Sung-Jun Hong,Byung Kwon Jung,Ihsan Ullah,Jong-Guk Kim,Jae-Ho Shin +7 more
TL;DR: Metagenomic profiles for both traditional and improved soil balls revealed that the various xenobiotic biodegradation, such as those for caprolactam, atrazine, xylene, toluene, styrene, bisphenol, and chlorocyclohexane might be responsible for organic waste cleanup.
References
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Journal ArticleDOI
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
TL;DR: Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment.
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Quantitative monitoring of gene expression patterns with a complementary DNA microarray.
TL;DR: A high-capacity system was developed to monitor the expression of many genes in parallel by means of simultaneous, two-color fluorescence hybridization, which enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA.
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Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.
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Mass spectrometry-based proteomics
Ruedi Aebersold,Matthias Mann +1 more
TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
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Real time quantitative PCR.
TL;DR: Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.